Simultaneous Determination of 5 Active Components in Callicarpae caulis et Folium by High Performance Liquid Chromatography

WU Yingxin; WANG Rushang; LIN Mingyue

doi:10.14009/j.issn.1672-2124.2022.09.004

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Simultaneous Determination of 5 Active Components in Callicarpae caulis et Folium by High Performance Liquid Chromatography

更新时间：2023-04-16

- Simultaneous Determination of 5 Active Components in Callicarpae caulis et Folium by High Performance Liquid Chromatography
- Vol. 22, Issue 9, Pages: 1042-1045(2022)
- 作者机构：
  
  1. 佛山市中医院西药房
  2. 广州康臣药物研究有限公司研发中心
  3. 佛山市中医院制剂中心
- 作者简介：
- 基金信息：
- DOI：10.14009/j.issn.1672-2124.2022.09.004
  CLC： R284.1
- Published：2022，
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WU Yingxin, WANG Rushang, LIN Mingyue. Simultaneous Determination of 5 Active Components in Callicarpae caulis et Folium by High Performance Liquid Chromatography. [J]. 22(9):1042-1045(2022)
DOI：

WU Yingxin, WANG Rushang, LIN Mingyue. Simultaneous Determination of 5 Active Components in Callicarpae caulis et Folium by High Performance Liquid Chromatography. [J]. 22(9):1042-1045(2022) DOI： 10.14009/j.issn.1672-2124.2022.09.004.

摘要

目的：建立同时测定广东紫珠中5种有效成分的高效液相色谱法(HPLC)。方法：采用ZORBAX Extend-C

色谱柱(4.6 mm×250 mm

5μm)

流动相为乙腈-0.1%乙酸水，梯度洗脱，流速为1 mL/min

检测波长为330 nm

柱温为35℃。结果：连翘酯苷B、金石蚕苷、毛蕊花糖苷、木犀草苷及木犀草素分别在18.57～371.40μg/mL(r=0.999 7)、15.33～306.60μg/mL(r=0.999 9)、5.62～112.40μg/mL(r=0.999 7)、1.29～25.80μg/mL(r=0.999 8)及3.54～70.80μg/mL(r=0.999 7)范围内线性关系良好，平均加样回收率为98.98%～100.20%。结论：本研究建立了同时测定广东紫珠5种成分的HPLC法，该方法快捷简便、重复性良好、结果准确可靠，可为广东紫珠药材质量标准提升提供参考依据。

Abstract

OBJECTIVE: To establish the high performance liquid chromatography（HPLC） method for simultaneous determination of 5 active components in Callicarpae caulis et Folium. METHODS: ZORBAX Extend-C

column（4.6 mm×250 mm

5 μm） was adopted

the mobile phase was acetonitrile and 0.1% acetic acid with gradient elution

the flow rate was 1 mL/min

the detection wavelength was 330 nm and the column temperature was 35 ℃. RESULTS: Forsythiaside B

poliumoside

verbascoside

galuteolin and luteolin from 18.57 to 371.40 μg/mL（r=0.999 7）

from 15.33 to 306.60 μg/mL（r=0.999 9）

from 5.62 to 112.40 μg/mL（r=0.999 7）

from 1.29 to 25.80 μg/mL（r=0.999 8） and from 3.54 to 70.80 μg/mL（r=0.999 7） were with a good linear

the average recoveries were from 98.98% to 100.20%. CONCLUSIONS: The established HPLC method for simultaneous determination of 5 active components in Callicarpae caulis et Folium is simple

rapid

reproducible

accurate and reliable

and can be used to control the quality standard of Callicarpae caulis et Folium.

关键词

广东紫珠高效液相色谱法有效成分含量

Keywords

Callicarpae caulis et FoliumHigh performance liquid chromatographyActive componentsContent

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