Effects of Dezocine on the Proliferation and Differentiation of Osteoblasts MC3T3-E1 and the Wnt/β-Catenin Signal Pathway

SONG Linlin; SUN Na; WANG Zhensheng; WEI Xinyun

doi:10.14009/j.issn.1672-2124.2022.08.010

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Effects of Dezocine on the Proliferation and Differentiation of Osteoblasts MC3T3-E1 and the Wnt/β-Catenin Signal Pathway

更新时间：2023-04-16

- Effects of Dezocine on the Proliferation and Differentiation of Osteoblasts MC3T3-E1 and the Wnt/β-Catenin Signal Pathway
- Vol. 22, Issue 8, Pages: 939-943(2022)
- 作者机构：
  
  1. 衡水市第二人民医院麻醉科
  2. 衡水市第二人民医院骨科
- 作者简介：
- 基金信息：
- DOI：10.14009/j.issn.1672-2124.2022.08.010
  CLC： R285
- Published：2022，
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SONG Linlin, SUN Na, WANG Zhensheng, et al. Effects of Dezocine on the Proliferation and Differentiation of Osteoblasts MC3T3-E1 and the Wnt/β-Catenin Signal Pathway. [J]. 22(8):939-943(2022)
DOI：

SONG Linlin, SUN Na, WANG Zhensheng, et al. Effects of Dezocine on the Proliferation and Differentiation of Osteoblasts MC3T3-E1 and the Wnt/β-Catenin Signal Pathway. [J]. 22(8):939-943(2022) DOI： 10.14009/j.issn.1672-2124.2022.08.010.

摘要

目的：探讨地佐辛对成骨细胞MC3T3-E1增殖、分化及对Wnt/β-catenin信号通路的影响。方法：使用质量浓度为0、5、10、20、40和80μg/mL的地佐辛处理MC3T3-E1细胞48 h后，采用细胞计数试剂盒-8(CCK-8)检测细胞存活率，计算半数抑制浓度(IC

)。将MC3T3-E1细胞分为对照组、地佐辛低浓度组(10μg/mL)、地佐辛中浓度组(20μg/mL)、地佐辛高浓度组(40μg/mL)和地佐辛高浓度+氯化锂(LiCl)组(40μg/mL+20μmol/L)

采用CCK-8检测细胞存活率；碱性磷酸酶(ALP)检测试剂盒检测细胞ALP活性；茜素红染色检测细胞钙化结节；蛋白质印迹法检测细胞中成骨分化相关蛋白[Runt相关转录因子2(RUNX2)、骨桥蛋白(OPN)和骨钙素(OCN)]及Wnt/β-catenin信号通路蛋白(Wnt3a、β-catenin)表达水平。结果：MC3T3-E1细胞对地佐辛的IC

约为20μg/mL;与对照组比较，地佐辛低、中及高浓度组细胞存活率、ALP活性和钙化结节数目，RUNX2、OPN、OCN、Wnt3a和β-catenin蛋白表达水平依次降低，差异均有统计学意义(P

0.05);与地佐辛高浓度组比较，地佐辛高浓度+LiCl组细胞存活率、ALP活性和钙化结节数目，RUNX2、OPN、OCN、Wnt3a和β-catenin蛋白表达水平明显升高，差异均有统计学意义(P

0.05)。结论：地佐辛可通过抑制Wnt/β-catenin信号通路来抑制成骨细胞MC3T3-E1的增殖、分化。

Abstract

OBJECTIVE: To probe into the effects of dezocine on the proliferation and differentiation of osteoblasts MC3 T3-E1 and the Wnt/β-catenin signaling pathway. METHODS: MC3 T3-E1 cells were treated with dezocine at concentrations of 0

40 and 80 μg/mL for 48 hours

cell counting kit-8（CCK-8） was used to detect the cell survival rate and calculate the half inhibitory concentration(IC

). MC3 T3-E1 cells were divided into control group

dezocine low concentration group（10 μg/mL）

dezocine medium concentration group（20 μg/mL）

dezocine high concentration group（40 μg/mL） and dezocine high concentration+ lithium chloride（LiCl） group（40 μg/mL+20 μmol/L）

the CCK-8 was used to detect cell survival rate; alkaline phosphatase（ALP） detection kit was used to detect the ALP activity; the alizarin red staining was used to detect cellular calcified nodules; the Western blot was used to detect the expression levels of osteogenic differentiation related protein [Runt-related transcription factor 2（RUNX2）

osteopontin（OPN）

osteocalcin（OCN）] and Wnt/β-catenin pathway related proteins（Wnt3 a

β-catenin） in the cells. RESULTS: The IC

of MC3 T3-E1 cells to dezocine was about 20 μg/mL; compared with the control group

the cell survival rate

ALP activity

number of calcified nodules

expression levels of RUNX2

OPN

OCN

Wnt3 a and β-catenin protein in dezocine low

medium and high concentration groups were decreased successively

with statistically significant difference（P

0.05）; compared with dizocine high concentration group

the cell survival rate

ALP activity

number of calcified nodules

the expression levels of RUNX2

OPN

OCN

Wnt3 a and β-catenin protein in dezocine high concentration+LiCl group were significantly higher

with statistically significant difference（P

0.05）. CONCLUSIONS: Dezocine can inhibit the proliferation and differentiation of osteoblasts MC3 T3-E1 by inhibiting the Wnt/β-catenin signaling pathway.

关键词

地佐辛成骨细胞MC3T3-E1增殖分化Wnt/β-catenin信号通路

Keywords

DezocineOsteoblasts MC3T3-E1ProliferationDifferentiationWnt/β-catenin signaling pathway

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