OBJECTIVE: To investigate the mechanism of Qigui Tongluo oral liquid in the treatment of transient ischemic attack（TIA） based on network pharmacology. METHODS: Chemical ingredients and target genes of 8 traditional Chinese medicines in Qigui Tongluo oral liquid were obtained by using Traditional Chinese medicine systems pharmacology database and analysis platform

encyclopedia of traditional Chinese medicine and literature search. Targets related to TIA were screened through GeneCards database and on-line Mendelian inheritance in man

the interactive targets of Qigui Tongluo oral liquid and TIA were further acquired. The interaction network of “single drug-active ingredient-target-disease” was constructed by Cytoscape software

and the protein-protein interaction（PPI） network was constructed by STRING database. WebGestalt enrichment analysis and DAVID database were used to conduct gene ontology（GO） functional enrichment analysis and Kyoto Encyclopedia of Genes and Genomes（KEGG） pathway enrichment analysis for related potential targets. Autodock software was used to molecularly dock the selected proteins with the active ingredients of Qigui Tongluo oral liquid. RESULTS: A total of 87 potential active ingredients and 40 potential targets related to TIA were screened out from Qigui Tongluo oral liquid

the main active ingredients included lumbrokinase

quercetin

β-sitosterol

luteolin and isoflavone. Key targets of Qigui Tongluo oral liquid in the treatment of TIA included ESR1

CASP3

F7

vascular endothelial growth factor A

PLG and P2 RY12. GO enrichment analysis showed that biological processes mainly involved multicellular organismal process

response to stimulus and biological regulation. KEGG pathway enrichment analysis showed 77 metabolic pathways

mainly included hypoxia-inducible factor 1 signaling pathway

Platelet activation signaling pathway

Rap1 signaling pathway

Fluid shear stress and atherosclerosis signaling pathway

etc. Molecular docking showed that the bioactive compounds had good binding activity with the protein receptors of key target genes. CONCLUSIONS: Results of network pharmacology reveals the characteristics of Qigui Tongluo oral liquid in the treatment of TIA with multiple ingredients

multiple targets and multiple pathways

and predicts its possible active ingredients

action pathways and key targets

providing theoretical basis for the study of pharmacodynamic substance basis and mechanism of action.