Effects of Ginsenoside Rh2 and Rg3 on the Proliferation and Invasion of Gastric Cancer Cells

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Effects of Ginsenoside Rh2 and Rg3 on the Proliferation and Invasion of Gastric Cancer Cells

更新时间：2022-10-08

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- Effects of Ginsenoside Rh2 and Rg3 on the Proliferation and Invasion of Gastric Cancer Cells
- Vol. 22, Issue 5, Pages: 539-543(2022)
- 作者机构：
  
  1. 重庆两江新区第一人民医院药剂科
  2. 重庆医科大学中医药学院
- 作者简介：
- 基金信息：
- DOI：10.14009/j.issn.1672-2124.2022.05.005
  CLC： R285
- Published：2022，
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LIU Yuanyuan, WANG Huan, ZHOU Ping, et al. Effects of Ginsenoside Rh2 and Rg3 on the Proliferation and Invasion of Gastric Cancer Cells. [J]. 22(5):539-543(2022)
DOI：

LIU Yuanyuan, WANG Huan, ZHOU Ping, et al. Effects of Ginsenoside Rh2 and Rg3 on the Proliferation and Invasion of Gastric Cancer Cells. [J]. 22(5):539-543(2022) DOI： 10.14009/j.issn.1672-2124.2022.05.005.

摘要

目的：探讨人参皂苷Rh2和Rg3在免疫微环境中通过调节程序性死亡蛋白配体1（PD-L1）对胃癌细胞增殖和侵袭的影响。方法：将胃癌细胞MGC-803分为三组，即对照组、Rh2+Rg3组（采用40μg/mL混合有人参皂苷Rh2和Rg3的培养基）和Rh2+Rg3+PD-L1组（将转染PD-L1载体质粒以3×10

3

的密度经过质量浓度为40μg/mL的人参皂苷Rh2和Rg3处理）。采用定量反转录聚合酶链反应和蛋白质印迹法检测PD-L1的表达；采用Annexin-V检测胃癌细胞MGC-803的凋亡情况；采用EdU方法检测胃癌细胞MGC-803的增殖情况；采用Transwell检测胃癌细胞MGC-803的迁移和侵袭情况。结果：Rh2+Rg3组的PD-L1 mRNA和蛋白的表达水平明显低于对照组，Rh2+Rg3+PD-L1组的PD-L1 mRNA和蛋白的表达水平明显高于Rh2+Rg3组，差异均有统计学意义（P

<

0.05）。Rh2+Rg3组的细胞凋亡率明显高于对照组，Rh2+Rg3+PD-L1组的细胞凋亡率明显低于Rh2+Rg3组，差异均有统计学意义（P

<

0.05）。Rh2+Rg3组的细胞克隆数量明显低于对照组，Rh2+Rg3+PD-L1组的细胞克隆数量明显高于Rh2+Rg3组，差异均有统计学意义（P

<

0.05）。Rh2+Rg3组的细胞增殖能力明显低于对照组，Rh2+Rg3+PD-L1的细胞增殖能力明显高于Rh2+Rg3组，差异均有统计学意义（P

<

0.05）。Rh2+Rg3组的迁移细胞数和侵袭细胞数明显少于对照组，Rh2+Rg3+PD-L1组的迁移细胞数和侵袭细胞数明显多于Rh2+Rg3组，差异均有统计学意义（P

<

0.05）。结论：人参皂苷Rh2和Rg3通过调节PD-L1的表达抑制胃癌肿瘤细胞的增殖、迁移和侵袭。

Abstract

OBJECTIVE: To probe into the effects of ginsenoside Rh2 and Rg3 on the proliferation and invasion of gastric cancer cells through regulating programmed death ligand-1（PD-L1）in the immune microenvironment. METHODS: Gastric cancer cells MGC-803 were divided into three groups

including control group

Rh2+Rg3 group（treated with 40 μg/mL ginsenoside Rh2 and Rg3） and Rh2+Rg3+PD-L1 group（transfected PD-L1 carrier vector was treated with 40 μg/mL ginsenoside Rh2 and Rg3 at a density of 3×10~3）. Quantitative reverse transcription polymerase chain reaction and Western blotting were used to detect the expression of PD-L1; Annexin-V was used to detect the apoptosis of MGC-803; EdU method was used to detect the proliferation of gastric cancer cells MGC-803; Transwell was used to detect the migration and invasion of gastric cancer cells MGC-803. RESULTS: The expression levels of PD-L1 mRNA and protein of the Rh2+Rg3 group were significantly lower than those of the control group

and the expression levels of PD-L1 mRNA and protein of the Rh2+Rg3+PD-L1 group were significantly higher than those of the Rh2+Rg3 group

with statistically significant differences（P<0.05）. The cell apoptosis rate of the Rh2+Rg3 group was significantly higher than that of the control group

and the cell apoptosis rate of the Rh2+Rg3+PD-L1 group was significantly lower than that of the Rh2+Rg3 group

with statistically significant differences（P<0.05）. The number of cell clones of the Rh2+Rg3 group was significantly less than that of the control group

and the number of cell clones of the Rh2+Rg3+PD-L1 group was significantly more than that of the Rh2+Rg3 group

with statistically significant differences（P<0.05）. The cell reproductive capacity of the Rh2+Rg3 group was significantly lower than that of the control group

and the cell reproductive capacity of the Rh2+Rg3+PD-L1 group was significantly higher than that of the Rh2+Rg3 group

with statistically significant differences（P<0.05）. The number of migrating and invading cells of the Rh2+Rg3 group was significantly less than that of the control group

and the number of migrating and invading cells of the Rh2+Rg3+PD-L1 group was significantly more than that of the Rh2+Rg3 group

with statistically significant differences（P<0.05）. CONCLUSIONS: Ginsenosides Rh2 and Rg3 can inhibit the proliferation

migration and invasion of gastric cancer cells by regulating the expression of PD-L1.

关键词

人参皂苷Rh2人参皂Rg3程序性死亡蛋白配体1胃癌增殖

Keywords

Ginsenoside Rh2Ginsenoside Rg3Programmed death ligand-1Gastric cancerProliferation

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