OBJECTIVE: To probe into the main chemical composition of Yinzhihuang granules and its pharmacological mechanism. METHODS: The chromatographic column was a Waters ACQUITY PRM UPLC BEH C

18

Column with Van Guard FIT（2.1 mm×100 mm

1.7 μm）

the mobile phase of gradient elution was 0.1% formic acid solution-acetonitrile; the high-resolution liquid mass spectrometry with positive and negative ion scanning mode（UHPLC-LTQ-Orbitrap-MS） was used. The target prediction was performed on detected chemical components by using the TCMSP and Swiss Target Prediction database

association network diagram was drawn in combination with Cytoscape 3.8.2

the gene ontology function enrichment analysis and Kyoto Encyclopedia of Gene and Genome pathway enrichment analysis on the core target gene were conducted

so that the component-target-pathway network diagram was constructed. Molecular docking verification of the disease targets and the main chemical components of Yinzhihuang granules was performed by using Autodock vina software. RESULTS: According to the results obtained from accurate relative molecular mass data and multilevel mass spectrometry fragment ion data

combined with the reference substance and literature reports

a total of 68 chemical components were analyzed and identified from Yinzhihuang granules

among which 14 chemical compounds were fully identified by reference substance

mainly including phenylpropanoids such as chlorogenic acid

coumarins such as scoparone

flavonoids such as baicalin and baicalin glycosides. Totally 31 main targets of Yinzhihuang for the treatment of jaundice obtained from network pharmacological analysis

and the top 5 main targets and important related signaling pathways were obtained after degree screening. It was inferred that Yinzhihuang granules may affect steroid hormone biosynthesis through lipid and atherosclerotic ways

thus treating the early lesions of jaundice. CONCLUSIONS: This study initially explored the main chemical constituents of Yinzhihuang granules and related pharmacological mechanisms

and provided a reference for further in-depth research of Yinzhihuang granules.