Effects of Ropivacaine on the Proliferation, Apoptosis, Invasion, Migration and RhoA/ROCK1 Signaling Pathway of Glioma U251 Cells

ZHANG Jinxia; LIANG Sujuan; YANG Hua; HUO Haoran

doi:10.14009/j.issn.1672-2124.2022.04.015

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Effects of Ropivacaine on the Proliferation, Apoptosis, Invasion, Migration and RhoA/ROCK1 Signaling Pathway of Glioma U251 Cells

更新时间：2022-10-08

- Effects of Ropivacaine on the Proliferation, Apoptosis, Invasion, Migration and RhoA/ROCK1 Signaling Pathway of Glioma U251 Cells
- Vol. 22, Issue 4, Pages: 453-456(2022)
- 作者机构：
  
  邯郸市中心医院神经外科
- 作者简介：
- 基金信息：
- DOI：10.14009/j.issn.1672-2124.2022.04.015
  CLC： R965
- Published：2022，
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ZHANG Jinxia, LIANG Sujuan, YANG Hua, et al. Effects of Ropivacaine on the Proliferation, Apoptosis, Invasion, Migration and RhoA/ROCK1 Signaling Pathway of Glioma U251 Cells. [J]. 22(4):453-456(2022)
DOI：

ZHANG Jinxia, LIANG Sujuan, YANG Hua, et al. Effects of Ropivacaine on the Proliferation, Apoptosis, Invasion, Migration and RhoA/ROCK1 Signaling Pathway of Glioma U251 Cells. [J]. 22(4):453-456(2022) DOI： 10.14009/j.issn.1672-2124.2022.04.015.

摘要

目的：探讨罗哌卡因对脑胶质瘤U251细胞增殖、凋亡、侵袭、迁移的作用及对Ras同源基因家族成员A(RhoA)/Rho相关卷曲螺旋蛋白激酶1(ROCK1)信号通路的影响。方法：将脑胶质瘤U251细胞分为阴性对照组、阳性对照组，罗哌卡因低、中及高剂量组。阴性对照组正常培养不进行处理，阳性对照组给予顺铂10μg/L

罗哌卡因低、中及高剂量组分别给予罗哌卡因25、50及100 mg/L。继续培养48 h后，采用四甲基噻唑蓝法、流式细胞仪、Transwell小室和划痕法检测细胞增殖率、凋亡率、细胞侵袭数和迁移距离，采用蛋白质印迹法检测细胞中RhoA、ROCK1和基质金属蛋白酶2(MMP-2)蛋白表达。结果：与阴性对照组比较，阳性对照组和罗哌卡因各剂量组脑胶质瘤U251细胞增殖率降低，细胞侵袭数减少，迁移距离缩短，RhoA、ROCK1和MMP-2蛋白表达降低，细胞凋亡率升高，差异均有统计学意义(P<0.05)

且罗哌卡因呈剂量依赖性。与阳性对照组比较，罗哌卡因各剂量组脑胶质瘤U251细胞增殖率升高，细胞侵袭数增加，迁移距离延长，RhoA、ROCK1和MMP-2蛋白表达升高，细胞凋亡率降低，差异均有统计学意义(P<0.05)。结论：罗哌卡因可以抑制脑胶质瘤U251细胞增殖、侵袭和迁移，促进脑胶质瘤U251细胞凋亡，其机制可能与抑制RhoA/ROCK1通路的表达有关。

Abstract

OBJECTIVE: To probe into the effects of ropivacaine on the proliferation

apoptosis

invasion

migration and the Ras homolog gene family member A（RhoA）/Rho-associated coiled-coil protein kinase 1（ROCK1） signaling pathway in glioma U251 cells. METHODS: Glioma U251 cells were divided into negative control group

positive control group

and ropivacaine low

medium and high dose groups. The negative control group was cultured normally without treatment

the positive control group was given 10 μg/L of cis-platinum

the ropivacaine low

medium and high dose groups were respectively given 25 mg/L

50 mg/L and 100 mg/L of ropivacaine. After 48 h of culture

the cell proliferation rate

apoptosis rate

cell invasion number and migration distance were detected by tetramethylthiazole blue method

flow cytometry

Transwell and scratch method

the expression of RhoA

ROCK1 and matrix metalloproteinase 2（MMP-2） protein were detected by Western blotting. RESULTS: Compared with the negative control group

the proliferation rate was decreased

the invasion number decreased

the migration distance was shortened

the expression of RhoA

ROCK1 and MMP-2 protein decreased

the apoptosis rate increased in the positive control and each dose group of ropivacain

with statistically significant differences（P<0.05）

and the ropivacaine was dose-dependent. Compared with the positive control group

the proliferation rate and the cell invasion number increased

the migration distance was prolonged

the expression of RhoA

ROCK1 and MMP-2 proteins increased

the apoptosis rate decreased in each dose group of ropivacaine

with statistically significant differences（P<0.05）. CONCLUSIONS: Ropivacaine can inhibit the proliferation

invasion and migration of glioma U251 cells

promote the apoptosis of glioma U251 cells

and the mechanism may be related to the inhibition of RhoA/ROCK1 pathway expression.

关键词

罗哌卡因脑胶质瘤U251细胞增殖凋亡侵袭迁移RhoA/ROCK1通路

Keywords

RopivacaineGlioma U251 cellsProliferationApoptosisInvasionMigrationRhoA/ROCK1 pathway

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