Effects of Compound Kushen Injection on Proliferation and Apoptosis of RPMI8226 Cells in Multiple Myeloma

LIU Rui; FAN Ningjian; WANG Yingman; SONG Jie; ZHANG Yan

doi:10.14009/j.issn.1672-2124.2022.03.011

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Effects of Compound Kushen Injection on Proliferation and Apoptosis of RPMI8226 Cells in Multiple Myeloma

更新时间：2022-10-08

- Effects of Compound Kushen Injection on Proliferation and Apoptosis of RPMI8226 Cells in Multiple Myeloma
- Vol. 22, Issue 3, Pages: 300-303(2022)
- 作者机构：
  
  1. 华北理工大学附属医院肿瘤放化疗科
  2. 唐山市第二医院脊柱三科
- 作者简介：
- 基金信息：
- DOI：10.14009/j.issn.1672-2124.2022.03.011
  CLC： R285
- Published：2022，
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LIU Rui, FAN Ningjian, WANG Yingman, et al. Effects of Compound Kushen Injection on Proliferation and Apoptosis of RPMI8226 Cells in Multiple Myeloma. [J]. 22(3):300-303(2022)
DOI：

LIU Rui, FAN Ningjian, WANG Yingman, et al. Effects of Compound Kushen Injection on Proliferation and Apoptosis of RPMI8226 Cells in Multiple Myeloma. [J]. 22(3):300-303(2022) DOI： 10.14009/j.issn.1672-2124.2022.03.011.

摘要

目的：探讨复方苦参注射液对多发性骨髓瘤RPMI8226细胞增殖和凋亡的影响。方法：体外培养多发性骨髓瘤RPMI8226细胞系，采用四甲基偶氮唑盐法检测不同浓度复方苦参注射液对细胞增殖的影响，采用流式细胞仪检测细胞周期和凋亡，采用酶联免疫吸附法检测白细胞介素6（IL-6）的表达水平。结果：0.5、1、2、5和10 mg/mL的复方苦参注射液均有抑制骨髓瘤RPMI8226细胞增殖的作用，且呈时间和剂量依赖性。5、10 mg/mL的复方苦参注射液可显著促进细胞凋亡，并阻滞细胞周期于G

期。复方苦参注射液治疗后，细胞IL-6表达水平明显下调。结论：复方苦参注射液能抑制骨髓瘤细胞的增殖，诱导凋亡，其机制可能与抑制IL-6的表达有关。

Abstract

OBJECTIVE: To probe into the effects of compound Kushen injection on proliferation and apoptosis of RPMI8226 cells in multiple myeloma. METHODS: RPMI8226 cells in multiple myeloma was subcultured in vitro. The effects of different concentrations of compound Kushen injection on cell proliferation were detected by Methyl thiazolyl tetrazolium

and cell cycle and apoptosis were detected by flow cytometry. The expression of interleukin-6（IL-6） was detected by enzyme-linked immunosorbent assay. RESULTS: Compound Kushen injection of 0.5 mg/mL

1 mg/mL

2 mg/mL

5 mg/mL and 10 mg/mL could inhibit the proliferation of RPMI8226 cells in multiple myeloma in a time and dose dependent manner. Compound Kushen injection of 5 mg/mL and 10 mg/mL could significantly promote cell apoptosis and block cell cycle in G

phase. The expression of IL-6 decreased significantly after the treatment of compound Kushen injection. CONCLUSIONS: Compound Kushen injection can inhibit the proliferation and induce the apoptosis of RPMI8226 cells

and its mechanism may be related to the inhibition of IL-6 expression.

关键词

多发性骨髓瘤复方苦参注射增殖白细胞介素6

Keywords

Multiple myelomaCompound Kushen injectionProliferationInterleukin-6

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