OBJECTIVE: To study the mechanism of rhubarb-leech-incense compatibility in treatment of endometriosis（EMT） based on network pharmacology. METHODS: TCMSP and BATMAN-TCM database were used to screen the active ingredients and target information of rhubarb-leech-incense. The target gene information of endometriosis was obtained through the treatment target database and GeneCards platform. Venny software was used to analyze the intersection of rhubarb-leeches-rhizome compatibility target gene and endometriosis target gene to obtain the core target of drugs. The “ingredient-target-disease” network was constructed by Cytoscape software

and the core target protein-protein interactions were analyzed by STRING database. Gene ontology（GO） functional enrichment analysis of core targets was performed by R software

and pathway enrichment analysis of Kyoto Encyclopedia of Genes and Genomes（KEGG） was performed by DAVID database. RESULTS: A total of 38 active ingredients and 98 potential core targets were screened. Quercetin

luteolin

kaempferol

safflower acid and ursolic acid were the main chemical constituents

and PTGS2

AR

PPARG

TOP2 A and ESR1 were the main targets. The main biological processes affected by rhubarb-leech-incense compatibility included lipopolysaccharide response

steroid hormone response and reactive oxygen species response. KEGG pathway enrichment analysis revealed 330 signaling pathways

mainly including PI3 K-Akt

HIF and TNF signaling pathways. CONCLUSIONS: Rhubarb-leech-incense can interact with PTGS2

AR and ESR1 through kaempferol

quercetin and luteolin to regulate PI3 K-Akt

HIF and TNF and other signaling pathways

affecting cell function

oxidative response and immune regulation

thus achieving a series of biological processes for the treatment of endometriosis.