Effects of Icariin on Proliferation,Migration,Apoptosis and PTEN/AKT Pathway of Renal Cancer OS-RC-2 Cells

MU Hongguang; MA Yuanyuan; YAO Shuqing; MA Yubin; REN Yujun; YANG Jingfang

doi:10.14009/j.issn.1672-2124.2021.11.013

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Effects of Icariin on Proliferation,Migration,Apoptosis and PTEN/AKT Pathway of Renal Cancer OS-RC-2 Cells

更新时间：2022-10-08

- Effects of Icariin on Proliferation,Migration,Apoptosis and PTEN/AKT Pathway of Renal Cancer OS-RC-2 Cells
- Vol. 21, Issue 11, Pages: 1339-1343(2021)
- 作者机构：
  
  1. 张家口宣钢医院药剂科
  2. 河北北方学院附属第二医院检验科
  3. 张家口宣钢医院肾病风湿免疫科
- 作者简介：
- 基金信息：
- DOI：10.14009/j.issn.1672-2124.2021.11.013
  CLC： R285
- Published：2021，
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MU Hongguang, MA Yuanyuan, YAO Shuqing, et al. Effects of Icariin on Proliferation,Migration,Apoptosis and PTEN/AKT Pathway of Renal Cancer OS-RC-2 Cells. [J]. 21(11):1339-1343(2021)
DOI：

MU Hongguang, MA Yuanyuan, YAO Shuqing, et al. Effects of Icariin on Proliferation,Migration,Apoptosis and PTEN/AKT Pathway of Renal Cancer OS-RC-2 Cells. [J]. 21(11):1339-1343(2021) DOI： 10.14009/j.issn.1672-2124.2021.11.013.

摘要

目的:探讨淫羊藿苷对肾癌OS-RC-2细胞增殖、迁移、凋亡的影响

以及对磷酸酶、张力蛋白同源物(PTEN)/蛋白激酶B(AKT)通路的影响。方法:设OS-RC-2细胞组、顺铂组(40μg/ml)和淫羊藿苷低、高浓度组(20、40μg/ml)。培养72 h后

测定各组肾癌OS-RC-2细胞存活率、迁移能力和凋亡率

PTEN、AKT的mRNA、蛋白表达水平。结果:与OS-RC-2细胞组比较

顺铂组和淫羊藿苷低、高浓度组细胞存活率降低

迁移距离缩短

PTEN、AKT的mRNA、蛋白表达水平降低

凋亡率升高

差异均有统计学意义(P <0.05)。与顺铂组比较

淫羊藿苷低浓度组细胞存活率升高

迁移距离延长

PTEN、AKT的mRNA、蛋白表达水平升高

凋亡率降低;淫羊藿苷高浓度组细胞存活率降低

迁移距离缩短

PTEN、AKT的mRNA、蛋白表达水平降低

凋亡率升高

差异均有统计学意义(P <0.05)。与淫羊藿苷低浓度组比较

淫羊藿苷高浓度组细胞存活率降低

迁移距离缩短

PTEN、AKT的mRNA、蛋白表达水平降低

凋亡率升高

差异均有统计学意义(P <0.05)。结论:淫羊藿苷能够抑制肾癌OS-RC-2细胞增殖和迁移

促进肾癌OS-RC-2细胞凋亡

其机制可能与淫羊藿苷降低肾癌OS-RC-2细胞PTEN、AKT的mRNA、蛋白表达

进而抑制PTEN/AKT通路的激活有关。

Abstract

OBJECTIVE: To probe into the effects of icariin on proliferation

migration

apoptosis and phosphatase and tensin homolog deleted on chromosome ten（ PTEN）/protein kinase B（ AKT） pathway of renal cancer OS-RC-2 cells. METHODS: OS-RC-2 cell group

cisplatin group（ 40 μg/ml）

icariin groups with different concentrations（ 20 μg/ml

40 μg/ml） were set up. After 72 h of culture

the survival rate

migration ability

apoptosis rate

and the mRNA and protein expression levels of PTEN and AKT were measured in each group of renal cancer OS-RC-2 cells. RESULTS: Compared with the OS-RC-2 cell group

the cell survival rate

migration distance

the mRNA and protein expression levels of PTEN and AKT decreased

and the apoptotic rate increased in the cisplatin group

icariin low-concentration group and icariin high-concentration group

with statistically significant differences（ P < 0. 05）.Compared with the cisplatin group

the cell survival rate

migration distance

the mRNA and protein expression levels of PTEN and AKT increased and the apoptotic rate decreased in the icariin low-concentration group; the cell survival rate

migration distance

the mRNA and protein expression levels of PTEN and AKT decreased and the apoptotic rate increased in the icariin high-concentration group

with statistically significant differences（ P < 0. 05）. Compared with the icariin low-concentration group

the cell survival rate

migration distance

the mRNA and protein expression levels of PTEN and AKT decreased and the apoptotic rate increased in the icariin high-concentration group

with statistically significant differences（ P < 0. 05）. CONCLUSIONS: Icariin can inhibit the proliferation and migration of renal cancer OS-RC-2 cells

and promote the apoptosis. The mechanism may be related to the reduction of the mRNA and protein expression levels of PTEN and AKT in renal cancer OS-RC-2 cells by icariin

which in turn inhibits the activation of PTEN/AKT pathway.

关键词

淫羊藿苷肾癌OS-RC-2细胞增殖迁移凋亡PTEN/AKT通路

Keywords

IcariinRenal cancer OS-RC-2 cellsProliferationMigrationApoptosisPTEN/AKT pathway

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