Quality Standard Improvement for Wuhuang Guwai Powder

SUN Yidong; ZHANG Meirong; ZHAN Yakun; ZHANG Hui; ZENG Xiangzhong; CHEN Baoting; ZHANG Sufang; LIN Aihua

doi:10.14009/j.issn.1672-2124.2021.11.007

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Quality Standard Improvement for Wuhuang Guwai Powder

更新时间：2022-10-08

- Quality Standard Improvement for Wuhuang Guwai Powder
- Vol. 21, Issue 11, Pages: 1313-1316(2021)
- 作者机构：
  
  1. 广东省中医院珠海医院药剂科
  2. 中山大学附属第五医院药学部
- 作者简介：
- 基金信息：
- DOI：10.14009/j.issn.1672-2124.2021.11.007
  CLC： R286.0
- Published：2021，
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SUN Yidong, ZHANG Meirong, ZHAN Yakun, et al. Quality Standard Improvement for Wuhuang Guwai Powder. [J]. 21(11):1313-1316(2021)
DOI：

SUN Yidong, ZHANG Meirong, ZHAN Yakun, et al. Quality Standard Improvement for Wuhuang Guwai Powder. [J]. 21(11):1313-1316(2021) DOI： 10.14009/j.issn.1672-2124.2021.11.007.

摘要

目的:提高乌黄骨外散的质量标准。方法:在现有质量标准的基础上

建立白芷、大黄的薄层色谱鉴定方法;建立乌头碱、次乌头碱含量测定的高效液相色谱法

色谱柱选用十八烷基硅烷键合硅胶色谱柱(Merck Purospher STAR RP-18 endcapped

250 mm×4.6 mm

5μm)

柱温为30℃

流速为0.8 ml/min。结果:大黄、白芷的薄层色谱斑点显色清晰

分离度好

阴性对照无干扰;乌头碱在1.00～6.00μg/ml(r=0.999 8)范围内线性良好

次乌头碱在15～90μg/ml(r=0.999 9)范围内线性良好

回收率分别为98.83%、99.87%

RSD分别为2.06%、2.63%。结论:该方法操作简便、准确可靠、重复性好

可用于乌黄骨外散的质量控制。

Abstract

OBJECTIVE: To improve the quality standard of Wuhuang Guwai powder. METHODS: Based on the previous quality standard

TLC identification method was established for radix angelicae dahuricae and radix et rhizoma rhei. HPLC method was established for content determination of aconitine and hypaconitine. The column was octadecylsilane chemically bonded silica gel column（ Merck Purospher STAR RP-18 endcapped

250 mm × 4. 6 mm

5 μm） at the flow rate of 0. 8 ml/min

the column temperature was 30 ℃ ． RESULTS: The TLC spots of radix angelicae dahuricae and radix et rhizoma rhei were clear and well separated without negative interference. The linearity was good in the range from 1. 00 to 6. 00 μg/ml（ r = 0. 999 8） for aconitine and from 15 to 90 μg/ml（ r = 0. 999 9）for hypaconitine

with the recovery of 98. 83% and 99. 87% and the RSD of 2. 06% and 2. 63%

respectively.CONCLUSIONS: The method is simple

accurate

reliable and repeatable

which can be used for the quality evaluation of the Wuhuang Guwai powder.

关键词

乌黄骨外散乌头碱次乌头碱质量标准薄层色谱法高效液相色谱法

Keywords

Wuhuang Guwai powderAconitineHypaconitineQuality standardTLCHPLC

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