OBJECTIVE: To probe into the mechanism of Huangqi Guizhi Wuwu decoction in rats with blood stasis syndrome. METHODS: The rats were divided into blank group

model group and administration group. The blood stasis model of rats was induced by subcutaneous injection of adrenaline combined with ice water bath（ model group and administration group）. The rats in the administration group were given Huangqi Guizhi Wuwu Decoction 8 g/kg by gavage once a day for 7 days

and the rats in the blank group and the model group were given the same amount of0. 9% sodium chloride solution by gavage once a day for 7 days. The blood coagulation index levels of the three groups were measured. A large amount of endogenous metabolite data were collected and processed by liquid chromatographyquadrupole-time of flight-mass spectrometry. Non-parametric univariate analysis and multivariate statistical analysis were used for the screening and identification of potential biomarkers. Metabolic pathways based on blood biomarkers were constructed to explore the pathogenesis of blood stasis syndrome and the mechanism of Huangqi Guizhi Wuwu decoction. RESULTS: Compared with the blank group

the TT

PT and APTT of the model group was shortened significantly

and the FIB content increased significantly

the difference was statistically significant（ P < 0. 01）.Compared with the model group

the TT

PT and APTT of the rats in the administration group was prolonged significantly

and the FIB content decreased significantly

the difference was statistically significant（ P < 0. 05）. The results of metabonomics showed that the intervention of Huangqi Guizhi Wuwu decoction on rats with blood stasis syndrome could cause substantial and characteristic changes of metabolic profile. Totally 10 metabolites were screened out

such as L-glutamic acid

L-phenylalanine and L-tryptophan; these metabolites returned to the normal levels after administration of Huangqi Guizhi Wuwu decoction. CONCLUSIONS: Huangqi Guizhi Wuwu decoction has a certain therapeutic effect on blood stasis syndrome. Regulation of metabolic pathways such as glutamic acid acid phenylalanine and tryptophan may be the mechanism of the treatment of blood stasis syndrome.