Protective Effects of Polydatin on Hyperoxic Lung Injury in Neonatal Mice

AN Min; WANG Shuaidao; YUAN Dongye; HE Liangnan

doi:10.14009/j.issn.1672-2124.2021.09.010

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Protective Effects of Polydatin on Hyperoxic Lung Injury in Neonatal Mice

更新时间：2022-10-08

- Protective Effects of Polydatin on Hyperoxic Lung Injury in Neonatal Mice
- Vol. 21, Issue 9, Pages: 1061-1064(2021)
- 作者机构：
  
  电子科技大学医学院附属绵阳医院·绵阳市中心医院烧伤整形外科
- 作者简介：
- 基金信息：
- DOI：10.14009/j.issn.1672-2124.2021.09.010
  CLC： R285.5
- Published：2021，
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AN Min, WANG Shuaidao, YUAN Dongye, et al. Protective Effects of Polydatin on Hyperoxic Lung Injury in Neonatal Mice. [J]. 21(9):1061-1064(2021)
DOI：

AN Min, WANG Shuaidao, YUAN Dongye, et al. Protective Effects of Polydatin on Hyperoxic Lung Injury in Neonatal Mice. [J]. 21(9):1061-1064(2021) DOI： 10.14009/j.issn.1672-2124.2021.09.010.

摘要

目的:研究虎杖苷通过SPOCK2表达对新生小鼠高氧肺损伤的保护作用。方法:体外培养小鼠正常肺上皮细胞mlE-12

作为对照组;利用高氧刺激mlE-12细胞

作为高氧组。用50、100和150μmol/ml的虎杖苷处理mlE-12细胞

再用高氧刺激

作为高氧+低剂量组、高氧+中剂量组和高氧+高剂量组。将pcDNA、pcDNA-SPOCK2转染至mlE-12细胞

再用高氧刺激细胞

作为高氧+pcDNA组、高氧+pcDNA-SPOCK2组。将si-NC、si-SPOCK2转染至mlE-12细胞

高氧刺激后

再用高剂量(150μmol/ml)虎杖苷处理细胞

作为高氧+高剂量+si-NC组、高氧+高剂量+si-SPOCK2组。采用化学法检测细胞丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性;采用酶联免疫吸附试验法检测细胞肿瘤坏死因子α(TNF-α)、白细胞介素1β(IL-1β)和白细胞介素6(IL-6)的含量。采用实时荧光定量聚合酶链反应检测细胞SPOCK2 mRNA表达。采用蛋白质印迹法检测SPOCK2蛋白质表达。结果:与对照组比较

高氧组细胞MDA含量显著升高

SOD活性显著降低;与高氧组比较

高氧+低剂量组、高氧+中剂量组和高氧+高剂量组细胞MDA含量显著降低

SOD活性显著升高

差异均有统计学意义(P<0.05)。与对照组比较

高氧组细胞TNF-α、IL-1β和IL-6水平显著升高;与高氧组比较

高氧+低剂量组、高氧+中剂量组和高氧+高剂量组细胞TNF-α、IL-1β和IL-6水平显著降低

差异均有统计学意义(P<0.05)。与对照组比较

高氧组细胞SPOCK2 mRNA和蛋白表达显著降低;与高氧组比较

高氧+低剂量组、高氧+中剂量组和高氧+高剂量组细胞SPOCK2 mRNA和蛋白表达显著升高

差异均有统计学意义(P<0.05)。与高氧+pcDNA组比较

高氧+pcDNA-SPOCK2细胞MDA含量和TNF-α、IL-1β、IL-6水平显著降低

SOD活性显著升高;与高氧+高剂量+si-NC组比较

高氧+高剂量+si-SPOCK2组细胞MDA含量和TNF-α、IL-1β、IL-6水平显著升高

SOD活性显著降低

差异均有统计学意义(P<0.05)。结论:虎杖苷通过上调SPOCK2表达抑制新生小鼠高氧肺损伤

对肺部具有保护作用。

Abstract

OBJECTIVE To probe into the protective effects of polydatin on hyperoxic lung injury through the expression of SPOCK2 in neonatal mice. METHODS: Normal lung epithelial cells mlE-12 in mice were cultured in vitro and set as the control group; mlE-12 cells were stimulated with hyperoxia and set as the hyperoxia group. The mlE-12 cells were treated with 50

100 and 150 μmol/ml of thujaplicin

and then stimulated with hyperoxia

which were set as the hyperoxia + low dose group

hyperoxia + medium dose group and hyperoxia + high dose group

respectively. The pcDNA and pcDNA-SPOCK2 were transfected into mlE-12 cells

which were stimulated with hyperoxia and set as hyperoxia + pcDNA group and hyperoxia + pcDNA-SPOCK2 group. The si-NC and si-SPOCK2 were transfected into mlE-12 cells

then stimulated with hyperoxia and treated with high dose（ 150 μmol/ml） of thujaplicin

which were set as hyperoxia + high dose + si-NC group and hyperoxia + high dose + si-SPOCK2 group.Malondialdehyde（ MDA） content and superoxide dismutase（ SOD） activity were detected by chemical method; tumor necrosis factor α（ TNF-α）

interleukin-1β（ IL-1β） and interleukin-6（ IL-6） content were detected by enzyme-linked immunosorbent assay. Real-time fluorescence quantitative polymerase chain reaction was used to detect SPOCK2 mRNA expression. Expression of SPOCK2 protein was detected by protein blotting. RESULTS: Compared with the control group

the MDA content increased significantly and SOD activity decreased significantly in the hyperoxia group; compared with the hyperoxia group

the MDA content decreased significantly and SOD activity increased significantly in the hyperoxia + low dose group

hyperoxia + medium dose group and hyperoxia + high dose group

with statistically significant differences（ P<0. 05）. Compared with the control group

the levels of TNF-α

IL-1β and IL-6 increased significantly in the hyperoxia group; compared with the hyperoxia group

the levels of TNF-α

IL-1β and IL-6 decreased significantly in the hyperoxia + low dose group

hyperoxia + medium dose group and hyperoxia + high dose group

with statistically significant differences（ P<0. 05）. Compared with the control group

the SPOCK2 mRNA and protein expression decreased significantly in the hyperoxia group; compared with the hyperoxia group

the SPOCK2 mRNA and protein expression increased significantly in the hyperoxia + low dose group

hyperoxia + medium dose group and hyperoxia + high dose group

with statistically significant differences（ P < 0. 05）. Compared with the hyperoxia + pcDNA group

the MDA content

levels of TNF-α

IL-1β and IL-6 decreased significantly and the SOD activity increased significantly in the hyperoxia + pcDNA-SPOCK2 cells; compared with the hyperoxia + high dose +si-NC group

the MDA content

levels of TNF-α

IL-1β and IL-6 increased significantly and SOD activity decreased significantly in the hyperoxia + high dose + si-SPOCK2 group

with statistically significant differences（ P < 0. 05）.CONCLUSIONS: Polydatin can inhibit hyperoxic lung injury in neonatal mice through up-regulating the SPOCK2 expression

which has protective effects for lung tissues.

关键词

虎杖苷SPOCK2高氧环境氧化应激炎症因子

Keywords

PolydatinSPOCK2HyperoxiaOxidative stressInflammatory factors

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