Effects of Simvastatin on T Cell Subsets and Expression of Inflammatory Factors in Peripheral Blood of Rats with Rib Fracture

LI Jian; WEN Boquan; GUO Shibing; WANG Yan

doi:10.14009/j.issn.1672-2124.2021.09.007

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Effects of Simvastatin on T Cell Subsets and Expression of Inflammatory Factors in Peripheral Blood of Rats with Rib Fracture

更新时间：2022-10-08

- Effects of Simvastatin on T Cell Subsets and Expression of Inflammatory Factors in Peripheral Blood of Rats with Rib Fracture
- Vol. 21, Issue 9, Pages: 1049-1052(2021)
- 作者机构：
  
  1. 开滦总医院林西医院麻醉科
  2. 唐山市中心医院麻醉科
- 作者简介：
- 基金信息：
- DOI：10.14009/j.issn.1672-2124.2021.09.007
  CLC： R965
- Published：2021，
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LI Jian, WEN Boquan, GUO Shibing, et al. Effects of Simvastatin on T Cell Subsets and Expression of Inflammatory Factors in Peripheral Blood of Rats with Rib Fracture. [J]. 21(9):1049-1052(2021)
DOI：

LI Jian, WEN Boquan, GUO Shibing, et al. Effects of Simvastatin on T Cell Subsets and Expression of Inflammatory Factors in Peripheral Blood of Rats with Rib Fracture. [J]. 21(9):1049-1052(2021) DOI： 10.14009/j.issn.1672-2124.2021.09.007.

摘要

目的:探讨辛伐他汀对肋骨骨折大鼠外周血T细胞亚型及炎症因子表达的影响。方法:建立大鼠肋骨骨折模型（n=60）

将大鼠分为对照组和辛伐他汀低、高剂量组。采用X光活体成像检测骨骼愈合状况;采用流式细胞仪检测大鼠外周血CD4

、CD8

T淋巴细胞含量

计算CD4

/CD8

;采用酶联免疫吸附法检测大鼠外周血肿瘤坏死因子α（TNF-α）、白细胞介素17（IL-17）水平的变化。结果:辛伐他汀低、高剂量组大鼠骨骼愈合程度优于对照组。与对照组相比

给药第1、2周辛伐他汀高剂量组、低剂量组大鼠的CD4

、CD8

T淋巴细胞含量和CD4

/CD8

比值明显升高

差异有统计学意义（P

0.05）;但辛伐他汀低剂量组与高剂量组大鼠之间的差异无统计学意义（P

0.05）。给药第3、4周

三组大鼠外周血CD4

、CD8

T淋巴细胞含量和CD4

/CD8

比值的差异均无统计学意义（P

0.05）。与对照组相比

辛伐他汀低、高剂量组大鼠在给药第1、2周的外周血TNF-α、IL-17水平明显升高

差异均有统计学意义（P

0.01）。给药第3、4周

三组大鼠外周血TNF-α、IL-17水平的差异均无统计学意义（P

0.05）。结论:辛伐他汀可影响大鼠骨折早期免疫细胞平衡

增加骨折早期免疫细胞因子TNF-α和IL-17的释放

促进早期骨骼愈合。

Abstract

OBJECTIVE: To explore the effects of simvastatin on T cell subsets and expression of inflammatory factors in peripheral blood of rats with rib fracture. METHODS: The rat rib fracture model was established（ n = 60）

and the rats were divided into the control group and low-dose and high-dose simvastatin group. X-ray in vivo imaging was used to detect bone healing. CD4

T lymphocytes and CD8

T lymphocytes in peripheral blood of rats were detected by flow cytometry

and CD4

/CD8

was calculated. Enzyme-linked immunosorbent assay was used to detect the changes of levels of tumor necrosis factor-α（ TNF-α） and interleukin 17（ IL-17） in the peripheral blood of rats.RESULTS: Compared with the control group

the bone healing degree of the low-dose and high-dose simvastatin group were better than those of the control group. The contents of CD4

T lymphocytes and CD8

T lymphocytes and the ratio of CD4

/CD8

in high-dose and low-dose simvastatin group were significantly higher than those in the control group at the 1 st and 2 nd week of administration

with statistically significant difference（ P

0. 05）. There was no significant difference between the low-dose simvastatin group and the high-dose simvastatin group（ P

0. 05）. At the 3 rd and 4 th week of administration

there was no significant difference in the content of CD4

T lymphocytes

CD8

T lymphocytes and the ratio of CD4

/CD8

in the peripheral blood of three groups of rats（ P

0. 05）. Compared with the control group

the levels of TNF-α and IL-17 in peripheral blood of rats in the low-dose and high-dose simvastatin group increased significantly at the 1 st and 2 nd week of administration

and the differences were statistically significant（ P

0. 01）. At the 3 rd and 4 th week of administration

there was no significant difference in the levels of TNF-α and IL-17 in the peripheral blood of three groups of rats（ P

0. 05）. CONCLUSIONS: Simvastatin can affect the balance of immune cells in the early stage of fracture in rats

increase the release of immune cytokines TNF-α and IL-17 in the early stage of fracture

and promote early bone healing.

关键词

辛伐他汀骨折T细胞炎症因子

Keywords

SimvastatinFractureT cellsInflammatory factors

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