Establishment of High Performance Liquid Chromatography Fingerprint of Shengjiang Xiexin Decoction

ZHANG Cong’en; WANG Huanjun; YU Xiaohong; ZHANG Xueqiang; LI Yue; ZHAO Kuijun; MA Zhijie

doi:10.14009/j.issn.1672-2124.2021.08.014

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Establishment of High Performance Liquid Chromatography Fingerprint of Shengjiang Xiexin Decoction

更新时间：2022-10-08

- Establishment of High Performance Liquid Chromatography Fingerprint of Shengjiang Xiexin Decoction
- Vol. 21, Issue 8, Pages: 955-958(2021)
- 作者机构：
  
  首都医科大学附属北京友谊医院中药剂科
- 作者简介：
- 基金信息：
- DOI：10.14009/j.issn.1672-2124.2021.08.014
  CLC： R284
- Published：2021，
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ZHANG Cong’en, WANG Huanjun, YU Xiaohong, et al. Establishment of High Performance Liquid Chromatography Fingerprint of Shengjiang Xiexin Decoction. [J]. 21(8):955-958(2021)
DOI：

ZHANG Cong’en, WANG Huanjun, YU Xiaohong, et al. Establishment of High Performance Liquid Chromatography Fingerprint of Shengjiang Xiexin Decoction. [J]. 21(8):955-958(2021) DOI： 10.14009/j.issn.1672-2124.2021.08.014.

摘要

目的:建立生姜泻心汤高效液相色谱指纹图谱分析方法

为生姜泻心汤的质量控制和临床应用提供参考。方法:收集生姜泻心汤组方药味的不同批次饮片

按药材产地和批号排列组合制备得到10批生姜泻心汤;采用高效液相色谱法对生姜泻心汤水煎液进行分析

并进行指纹图谱分析方法学考察;采用"中药色谱指纹图谱相似度评价系统(2004 A版)"进行指纹图谱的分析

并通过主要化学成分对照品进行共有峰的指认。结果:指纹图谱方法学研究结果显示

精密度、重复性及稳定性考察结果中

参照峰保留时间的RSD均<2%

参照峰面积的RSD均<5%;共标定出41个具有较好分离度的共有峰

通过对照品指认了其中6-姜辣素、绿原酸等9个共有峰; 10批生姜泻心汤相似度>0.99。结论:本研究建立的指纹图谱方法具有良好的精密度、重复性和稳定性

可为生姜泻心汤的质量控制、药理学研究等提供依据。

Abstract

OBJECTIVE: To establish the chromatographic fingerprint by high performance liquid chromatography（ HPLC） for Shengjiang Xiexin decoction

so as to provide reference for quality control and clinical application of Shengjiang Xiexin decoction. METHODS: Different batches of herbal decoction pieces as ingredients of Shengjiang Xiexin decoction were collected. Ten batches of Shengjiang Xiexin decoction were prepared according to the origin area and batch number. The decoction of Shengjiang Xiexin decoction was analyzed by HPLC

and the fingerprint analysis method was investigated. The fingerprints were analyzed by he Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine（ 2004 A）

and the common peaks were identified by the main chemical components of the control. RESULTS: The accuracy

reproducibility and stability test showed that the RSD of common peaks’ retention time（ RT） and peak area（ PA） were <2% and <5%

respectively. Totally 41 well separated common peaks were found in the fingerprints

in which 9 common peaks were identified as 6-gingerol and chlorogenic acid. The similarity of 10 batches of Shengjiang Xiexin decoction was > 0. 99. CONCLUSIONS: The fingerprint method established in the study has good precision

reproducibility and stability

which can provide basis for the quality control and pharmacological study of Shengjiang Xiexin decoction.

关键词

生姜泻心汤高效液相色谱指纹图谱分析共有峰

Keywords

Shengjiang Xiexin decoctionHigh performance liquid chromatographyFingerprint analysisCommon peaks

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