OBJECTIVE: To probe into the mechanism of Liangge Powder inhibiting cytokine storm in acute lung injury（ ALI） based on network pharmacology and molecular docking. METHODS: The active ingredients and action targets of Liangge Powder were screened from the traditional Chinese medicine systems pharmacology database and analysis platform; the disease targets of ALI were obtained from online mendelian inheritance in man

Gene Cards and other medical databases. Uniprot database was used to query the genes corresponding to the targets

R software and String database were adopted to construct protein-protein interaction（ PPI） network

Cytoscape software was used to construct herbal-compound-target interaction network

R software was used to perform gene ontology（ GO） function enrichment analysis and Kyoto encyclopedia of genes and genomes（ KEGG） pathway enrichment analysis

so as to predict the molecular mechanisms. Autodock Vina software was adopted to conduct molecular docking on the core components of Liangge Powder with IL-1β

IL-6

TNF-α receptors and glycogen synthase kinase 3β. RESULTS:Totally 149 active ingredients and 237 targets were found in Liangge Powder in the treatment of ALI

the key targets involving AKT1

IL-6 and MAPK3

etc.; the GO functional enrichment was mainly biological processes

including oxidative stress

lipopolysaccharide response and bacteriogenic molecule

etc. The KEGG pathway mainly involved PI3 K-Akt

AGE-RAGE and TNF pathways

etc.; results of molecular docking showed that the binding forces of core compounds

such as phillyrin

stigmasterol

glabridin

pterocarpin and quercetin

was similar to that of clinically used anti-inflammatory drugs

among which phillyrin was the strongest. CONCLUSIONS: Liangge Powder may act on targets and pathways of AKT1

IL-6

PI3 K-Akt and AGE-RAGE through various active ingredients such as phillyrin and and quercetin

and exert therapeutic effects on ALI in terms of anti-inflammatory

antibacterial

antioxidant and immunoregulation.