OBJECTIVE: To study the protective effect of herbal compound 861 on acute liver injury induced by carbon tetrachloride（ CCl4） and D-galactosamine（ D-GalN） in mice

and observe the main poisoning symptoms in mice

so as to provide reference for rational drug use in clinic. METHODS: Acute mice models of liver injury were induced by CCl4（ 0. 02 ml/kg） and D-GalN（ 0. 8 g/kg）. Biphenyl diester（ 0. 029 g/kg） was used as the positive drug. The effect and protection of liver of herbal compound 861（ 1. 5

3 and 6 g/kg） on serum alanine aminotransferase（ ALT） and aspertate aminotransferase（ AST） were observed. The mice were administered at the maximum dosing volume（ 1. 0 ml） and maximum drug concentration（ 60 g/kg）

and the symptoms of poisoning and death were recorded within 14 d. RESULTS: Herbal compound 861 had the strong inhibitory effect on the elevation of serum ALT and AST in mice with acute liver injury induced by single intrapitoneal injection of CCl4 and D-GalN. For CCl4-induced liver injury mice

there were statistically significant differences in ALT and AST levels in the high-and medium-dose group of herbal compound 861 compared with the model group（ P < 0. 05）. For D-GalN-induced liver injury mice

there were statistically significant differences in ALT and AST levels in the high-and medium-dose group of herbal compound 861 compared with the model group（ P < 0. 05）. Histological examination showed that herbal compound 861 had a certain protective effect on liver injury. No death occurred in mice under the condition of maximum dose and concentration of herbal compound 861. CONCLUSIONS: Herbal compound 861 has significant therapeutic effect on acute liver injury induced by CCl4 and D-GalN

and it is safe to use under the condition of maximum drug concentration.