OBJECTIVE: To probe into the effects of Zishen Tiaochong method and growth hormone on outcome of in vitro fertilization and embryo transfer（ IVF-ET） in women with diminished ovarian reserve（ DOR） at different age.METHODS: Pregnancy outcomes of 217 patients with DOR undergoing IVF-ET in Guangdong Women and Children Hospital from Jan. 2017 to Jan. 2019 were analyzed retrospectively

including 105 patients <35 years old and 112 patients≥35 years old. All patients were on antagonist regimens and were on the first cycle of superovulation. In each age group

according to different pretreatment methods

all patients were divided into the traditional Chinese medicine group

the GH group and the control group. The traditional Chinese medicine group was given intervention of Zishen Tiaochong method

the GH group was pretreated with GH

and the control group was not pretreated with TCM or GH. Differences in clinical and laboratory indexes and pregnancy outcomes were observed in the three groups within the two age groups.RESULTS: There was no significant change in ovarian response and clinical outcome in patients with DOR aged < 35 years old after GH intervention. The thickness of intrauterine membrane on the day of transplantation

number of harvested eggs and two prokaryotes（ 2 PN） fertilization rate of the traditional Chinese medicine group were slightly higher than those of the GH group and the control group

but the difference was not statistically significant（ P>0. 05）. For patients with DOR aged ≥35 years old

the total application time and total dose of Gonadotropin（ Gn） in the CH group were significanlt ly lower than those in the traditional Chinese medicine group and the control group; the optimal embryo rate in the GH group was significantly higher than that in the traditional Chinese medicine group and the control group

with statistically significant difference（ P<0. 05）. Regardless of patients aged ≥35 years old or aged <35 years old

the endometrial thickness of patients in the traditional Chinese medicine group was the lowest during menstruation

which was significantly different from the GH group and the control group（ P<0. 05）. Within the two age groups

there was no statistically significant difference in the implantation rate

pregnancy rate and clinical pregnancy rate in the traditional Chinese medicine group

CH group and the control group

the difference was not statistically significant（ P>0. 05）.CONCLUSIONS: The intervention of GH in the elderly（ ≥35 years old） patients with DOR can improve the ovarian reactivity to a certain extent

while Zishen Tiaochong method is more effective in younger（ <35 years old） patients with DOR

and Zishen Tiaochong method can promote the endometrium spallation during menstruation and increase the endometrium thickness on the day of transplantation.