OBJECTIVE: To probe into the target and mechanism of Menispermi Rhizoma and Typhonii Rhizoma in the treatment of non-small cell lung cancer based on network pharmacology. METHODS: Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform were used to screen the effective components of Menispermi Rhizoma and Typhonii Rhizoma

Swiss Target Prediction and Pharm Mapper were used to obtain compound targets.Online mendelian inheritance in man（ OMIM） database was used to screen disease gene network of non-small cell lung cancer

the network diagram of drug-component-target-disease was constructed

and protein network interaction

gene ontology（ GO） function analysis and Kyoto encyclopedia of genes and genomes（ KEGG） pathway analysis were carried out. RESULTS: The bioavailability ≥30% and drug like ≥0. 18 were selected as the screening conditions of effective components from menispermi rhizoma and typhonii rhizoma. A total of 13 compounds were obtained

and 363 human gene targets were obtained by analyzing the first 50 potential target protein genes of each compound. Through the analysis of the drug-component-target-disease interaction network

totally 101 targets on non-small cell lung cancer related genes were obtained. A total of 276 enrichment results were included in GO analysis

and 26 pathways in KEGG database were associated with non-small cell lung cancer. These results indicated that the combination of Menispermi Rhizoma and Typhonii Rhizoma has broad-spectrum anti-tumor properties

suggesting that they may play a role of treating with nonsmall cell lung cancer in the aspects of EGFR tyrosine kinase inhibitor resistance

angiogenesis

immune escape

apoptosis and regulation of tumor carbon metabolism. CONCLUSIONS: Based on the method of network pharmacology

the potential active components and potential targets of Menispermi Rhizoma and Typhonii Rhizoma in the prevention and treatment of non-small cell lung cancer are analyzed

which provides theoretical guidance and prediction for further study on the mechanism of compatibility of Menispermi Rhizoma and Typhonii Rhizoma.