Simultaneous Determination of Multi-Components in Wuling Capsules Based on Rapid Resolution Liquid-Chromatography High-Resolution Mass Spectrometry

LI Jun; GAO Hongjie; ZHANG Yan; LI Tao

doi:10.14009/j.issn.1672-2124.2021.02.008

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Simultaneous Determination of Multi-Components in Wuling Capsules Based on Rapid Resolution Liquid-Chromatography High-Resolution Mass Spectrometry

更新时间：2022-10-08

- Simultaneous Determination of Multi-Components in Wuling Capsules Based on Rapid Resolution Liquid-Chromatography High-Resolution Mass Spectrometry
- Vol. 21, Issue 2, Pages: 160-164(2021)
- 作者机构：
  
  1. 中国中医科学院西苑医院检验科
  2. 中国中医科学院中医药信息研究所党委办公室
  3. 南方医科大学中医药学院
  4. 中国中医科学院广安门医院心理科
- 作者简介：
- 基金信息：
- DOI：10.14009/j.issn.1672-2124.2021.02.008
  CLC： R286.0
- Published：2021，
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[1]李君,高宏杰,张艳,李涛.基于液相色谱-质谱联用技术同时测定乌灵胶囊多组分含量[J].中国医院用药评价与分析,2021,21(02):160-164.

LI Jun, GAO Hongjie, ZHANG Yan, et al. Simultaneous Determination of Multi-Components in Wuling Capsules Based on Rapid Resolution Liquid-Chromatography High-Resolution Mass Spectrometry. [J]. 21(2):160-164(2021)
[1]李君,高宏杰,张艳,李涛.基于液相色谱-质谱联用技术同时测定乌灵胶囊多组分含量[J].中国医院用药评价与分析,2021,21(02):160-164. DOI： 10.14009/j.issn.1672-2124.2021.02.008.

LI Jun, GAO Hongjie, ZHANG Yan, et al. Simultaneous Determination of Multi-Components in Wuling Capsules Based on Rapid Resolution Liquid-Chromatography High-Resolution Mass Spectrometry. [J]. 21(2):160-164(2021) DOI： 10.14009/j.issn.1672-2124.2021.02.008.

摘要

目的:建立基于液相色谱-质谱联用技术同时测定乌灵胶囊多组分含量的方法。方法:采用液相色谱-质谱联用技术

同时检测乌灵胶囊中30种组分的含量

包括腺苷、鸟苷、尿苷、腺苷酸、染料木素、甲基蜂蜜曲霉素、胆碱、左旋肉碱、多巴胺和21种氨基酸（甘氨酸、蛋氨酸、酪氨酸、组氨酸、苏氨酸、丙氨酸、亮氨酸、异亮氨酸、色氨酸、赖氨酸、天冬氨酸、天冬酰胺、缬氨酸、苯丙氨酸、脯氨酸、丝氨酸、谷氨酸、谷氨酰胺、精氨酸、牛磺酸及氨基丁酸）。色谱柱采用UPLC HSS T3柱（2.1 mm×100 mm

1.8μm）;流动相为乙腈-0.1%甲酸溶液

梯度洗脱;流速为0.35 ml/min;柱温为40℃。质谱采用电喷雾离子源正离子模式

飞行时间质量分析器全扫描结合信息依赖数据采集方法。结果:所有组分在20.0～800 ng/ml范围内线性关系良好（r>0.99）

加样回收率为87.6%～104.8%

日内、日间精密度的RSD<10.0%（n=6）。结论:该研究建立的方法可行

重复性好

可用于乌灵胶囊广谱内源性成分质量控制。

Abstract

OBJECTIVE: To establish the method for simultaneous determination of multi-components in Wuling Capsules based on rapid resolution liquid-chromatography high-resolution mass spectrometry. METHODS: Rapid resolution liquid-chromatography high-resolution mass spectrometry was adopted. Totally 30 components in Wuling capsules were detected

including adenosine

guanosine

uridine

adenylate

genistein

methylmellein

choline

L-carnitine

dopamine and 21 amino acids（ glycine

methionine

tyrosine

histidine

threonine

alanine

leucine

isoleucine

tryptophan

lysine

aspartic acid

asparagine

valine

phenylalanine

proline

serine

glutamine acid

glutamine

arginine

taurine and aminobutyric acid）. The chromatographic column was UPLC HSS T3（ 2. 1 mm ×100 mm

1. 8 μm）. The mobile phase was acetonitrile-0. 1% formic acid solution with gradient elution. The flow rate was 0. 35 ml/min and the column temperature was 40 ℃ . MS detection was performed with TOF full scan mode by using positive electro spray ionization. RESULTS: The linearity was achieved in the range from 20. 0 ng/ml to800 ng/ml for all the analyzed components（ r > 0. 99）. The average recovery rate of these compounds ranged from87. 6% to 104. 8%. The relative standard deviation of the precision experiment and the repeatability experiment was less than 10. 0%（ n = 6）. CONCLUSIONS: The established method is feasible and reproducible

and can be used for the quality control of high-profiling endogenous components of Wuling capsules.

关键词

乌灵胶囊多组分液相色谱-质谱联用技术含量测定

Keywords

Wuling capsulesMuli-compontensLiquid-chromatography high-resolution mass spectrometryContent determination

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