Inhibition of Fuzheng Qudu Prescription on the Human Breast Cancer MCF-7 Cell Proliferation and its Effects on PI3K/Akt Signaling Pathway and the Expression of Vascular Endothelial Growth Factor-C and Vascular Endothelial Growth Factor Receptor-3

SHI Hua; YANG Zhong; LI Ying

doi:10.14009/j.issn.1672-2124.2020.12.008

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Inhibition of Fuzheng Qudu Prescription on the Human Breast Cancer MCF-7 Cell Proliferation and its Effects on PI3K/Akt Signaling Pathway and the Expression of Vascular Endothelial Growth Factor-C and Vascular Endothelial Growth Factor Receptor-3

更新时间：2022-10-08

- Inhibition of Fuzheng Qudu Prescription on the Human Breast Cancer MCF-7 Cell Proliferation and its Effects on PI3K/Akt Signaling Pathway and the Expression of Vascular Endothelial Growth Factor-C and Vascular Endothelial Growth Factor Receptor-3
- Vol. 20, Issue 12, Pages: 1437-1441(2020)
- 作者机构：
  
  1. 北京中医医院延庆医院普外科
  2. 首都医科大学附属北京中医医院肿瘤科
- 作者简介：
- 基金信息：
- DOI：10.14009/j.issn.1672-2124.2020.12.008
  CLC： R285.5
- Published：2020，
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[1]时华,杨中,李颖.扶正祛毒方抑制人乳腺癌MCF-7细胞增殖及对PI3KAkt信号通路和血管内皮生长因子C、血管内皮细胞生长因子受体3表达的影响[J].中国医院用药评价与分析,2020,20(12):1437-1441.

SHI Hua, YANG Zhong, LI Ying. Inhibition of Fuzheng Qudu Prescription on the Human Breast Cancer MCF-7 Cell Proliferation and its Effects on PI3K/Akt Signaling Pathway and the Expression of Vascular Endothelial Growth Factor-C and Vascular Endothelial Growth Factor Receptor-3. [J]. 20(12):1437-1441(2020)
[1]时华,杨中,李颖.扶正祛毒方抑制人乳腺癌MCF-7细胞增殖及对PI3KAkt信号通路和血管内皮生长因子C、血管内皮细胞生长因子受体3表达的影响[J].中国医院用药评价与分析,2020,20(12):1437-1441. DOI： 10.14009/j.issn.1672-2124.2020.12.008.

SHI Hua, YANG Zhong, LI Ying. Inhibition of Fuzheng Qudu Prescription on the Human Breast Cancer MCF-7 Cell Proliferation and its Effects on PI3K/Akt Signaling Pathway and the Expression of Vascular Endothelial Growth Factor-C and Vascular Endothelial Growth Factor Receptor-3. [J]. 20(12):1437-1441(2020) DOI： 10.14009/j.issn.1672-2124.2020.12.008.

摘要

目的:探讨扶正祛毒方对人乳腺癌MCF-7细胞增殖的抑制作用及对PI3K/Akt信号通路和血管内皮生长因子C（VEGF-C）、血管内皮细胞生长因子受体3（VEGFR-3）表达的影响。方法:人乳腺癌细胞株MCF-7经完全培养基于37℃、5%CO2、完全饱和湿度培养箱中培养至对数生长周期

接种于96孔培养板

加入浓度梯度（5、10及20μmol/L）扶正祛毒方培养基

以正常培养细胞作为对照;孵育24 h后采用噻唑蓝法检测细胞增殖情况

计算细胞增殖抑制率;采用逆转录聚合酶链反应法检测VEGF-C、VEGFR-3 mRNA水平;采用蛋白质印迹法检测PI3K、AKT蛋白表达水平。结果:扶正祛毒方低、中及高剂量组细胞增殖抑制率明显高于对照组

差异均有统计学意义（P<0.05）

提示扶正祛毒方对乳腺癌细胞增殖具有较好的抑制效果

且随着剂量的增加抑制效果越明显;扶正祛毒方低、中及高剂量组细胞VEGF-C、VEGFR-3 mRNA水平明显低于对照组

差异均有统计学意义（P<0.05）

提示扶正祛毒方能够下调VEGF-C、VEGFR-3基因水平

且随着剂量的增加下调幅度越大;扶正祛毒方低、中及高剂量组细胞PI3K、Akt蛋白表达水平明显低于对照组

差异均有统计学意义（P<0.05）

且呈剂量依赖性

剂量越大降低幅度越明显。结论:扶正祛毒方能够抑制乳腺癌MCF-7细胞增殖

其机制可能与阻断PI3K/Akt信号通路

下调VEGF-C、VEGFR-3基因表达有关。

Abstract

OBJECTIVE:To probe into the inhibition of Fuzheng Qudu prescription on the human breast cancer MCF-7 cell proliferation and its effects on PI3 K/Akt signaling pathway and the expression of vascular endothelial growth factor-C（VEGF-C） and vascular endothelial growth factor receptor-3（VEGFR-3）.METHODS:The human breast cancer cell strain MCF-7 was cultured in the incubator of completely medium at 37 ℃

5% CO

fully saturated humidity to a logarithmic growth cycle

inoculated in a 96-well culture plate

added with concentration gradients（5

10 and 20 μmol/L） of Fuzheng Qudu prescription medium

set normal cultured cells as contrast;after 24 hours of incubation

the thiazole blue method was used to detect cell proliferation and calculate the cell proliferation inhibition rate;the reverse transcription polymerase chain reaction method was used to detect the mRNA levels of VECJF-C and VEGFR-3;Western Blot was used to detect the expression levels of PI3 K and AKT protein.RESULTS:The inhibition rate of cell proliferation in the low

medium and high dose groups of Fuzheng Qudu prescription was significantly higher than that of the control group

with statistically significant difference（P

0.05）

which suggested that Fuzheng Qudu prescription had a good inhibitory effect on breast cancer cell proliferation

and as the dose increase

the inhibitory effect more obvious:the mRNA levels of VFGF-C and VFGFR-3 in tne low

medium dose groups of the Fuzheng Qudu prescription were significantly lower than those of the control group

with statistically significant differences（P

0.05）

which suggested that Fuzheng Quclu prescription can down-regulate the VEGF-C and VEGFR-3 genes level

and as the dose increase

the decrease became greater;the expression levels of PI3 K and Akt protein in the low

medium and high-dose groups of Fuzheng Qudu prescription were significantly lower than those of the control group

with statistically significant differences（P

0.05）

which presented a dose-dependent

and the larger the dose

the more obvious the reduction.CONCLUSIONS:Fuzheng Qudu prescription can inhibit the cell proliferation of breast cancer MCF-7

its mechanism may be related to blocking PI3 K/Akt signaling pathway and down-regulating the expression of VEGF-C and VEGFR-3 genes.

关键词

乳腺癌增殖PI3K/Akt信号通路扶正祛毒方VEGF-CVEGFR-3

Keywords

Breast cancerProliferationPI3K/Akt signaling pathwayFuzheng Qudu prescriptionVEGF-CVEGFR-3

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