OBJECTIVE:To probe into the effects of Jiedu Xiao’ai formula on invasion

migration and epithelial mesenchymal transformation of glioma cells.METHODS:The glioma cell line SHG44 was cultured

and different concentrations（0

25

50

100 μg/ml

respectively

as blank control group

low-dose group

medium dose group and high-dose group） of the water extract of Jiedu Xiao ’ai formula were added to the medium.The cell scratch test was used to detect its influence on the migration ability

and Transwell invasion test was used to detect its influence on the cell invasion ability.Glioma cells were used to construct transplanted tumor in nude mice.After tumor formation

the mice were randomly divided into blank control group

low dose group

medium dose group and high-dose group

and fed with 0

25

50 and 100 μg/ml Jiedu Xiao ’ai formula

respectively.Western blot was used to detect the protein expression levels of ZEB1

snail

transforming growth factor(3（TGF-β） and matrix metalloproteinase 9（MMP-9） in transplanted tumors of nude mice.RESULTS:Cell scratch test showed that the migration distance of tumor cells in the low

medium and high dose group was significantly shorter than that in the blank control group after treatment with Jiedu Xiao’ai formula（P<0.05）

and the migration ability of cells was significantly inhibited.The inhibition of Jiedu Xiao’ai formula on glioma cell migration ability was time and concentration dependent.The longer the time and the higher the concentration

the weaker the cell migration ability.Transwell invasion experiment showed that the number of tumor cells invaded in the low

medium and high dose groups was significantly lower than that in the blank control group after being treated with Jiedu Xiao ’ai formula

the difference was statistically significant（P<0.05）

the cell invasion ability was significantly inhibited

and the inhibition of the glioma cell invasion ability of Jiedu Xiao ’ai formula was concentration dependent.The higher the concentration

the weaker the cell invasion ability.Western blot showed that the expressions of TGF-β

ZEB-1

snail and MMP-9 protein in glioma cells decreased significantly after being fed with Jiedu Xiao ’ai formula

and the low

medium and high dose groups were all lower than the blank control group

the differences were statistically significant（P<0.05）.The inhibition of the expression of TGF-β

ZEB-1

snail and MMP-9 by Jiedu Xiao’ai formula was concentration dependent.The higher the concentration

the lower the expression.CONCLUSIONS:Jiedu Xiao’ai formula can prevent the epithelial mesenchymal transformation of glioma cells

thereby inhibiting cell invasion and migration.Its mechanism of action is related to down-regulating the expression of various effector proteins such as TGF-β

ZEB1

snail and MMP-9 in cells.