OBJECTIVE: To establish a reversed phase high performance liquid chromatography（ RP-HPLC） to detect the contents of ephedrine hydrochloride

benzoyl aconine and oleanolic acid in Fengshigutong capsule.METHODS: Fengshigutong capsule sample was detected after extracted by acetonitrile-methanol（ V∶ V = 60∶ 40）

and the Shim-pack CLC ODS-C18 column was separated; the mobile phase A was acetonitrile-methanol（ V∶ V = 60∶ 40）

mobile phase B was 0. 1 mol/L ammonium acetate solution（ add 0. 5 ml glacial acetic acid per 1 000 ml）

gradient elution; with the detection wavelength was 221 nm

the flow rate was 1. 0 ml/min

the column temperature was 25 ℃

the sample volume was 25 μl; using external standard method. RESULTS: Ephedrine hydrochloride

benzoyl aconitine and oleanolic acid were separated well under this chromatographic condition

there was no interference peak in the negative sample solution; ephedrine hydrochloride

benzoyl aconitine and oleanolic acid had good linear relationship in the respective range of 0. 4-40. 0

0. 496-49. 6 and 0. 991-99. 1 μg/ml

with all the values of r > 0. 995; the average recovery rate of standard addition was 98. 50%

96. 94% and 98. 28%; the repeatability RSD was 4. 21%

3. 08% and 3. 17%

with good instrument precision. CONCLUSIONS: This method has the characteristics of simple sample processing

high detection efficiency and accurate results

which is suitable for the quality control of Fengshigutong capsules.