Determination of the Concentration of Micafungin in Human Plasma by HPLC and its Application in Critical Patients

ZHAO Mingming; CHEN Yanan; LU Tong; Li Guofei; ZHAO Limei

doi:10.14009/j.issn.1672-2124.2020.09.017

您当前的位置：

首页 >

文章列表页 >

Determination of the Concentration of Micafungin in Human Plasma by HPLC and its Application in Critical Patients

更新时间：2022-10-08

- Determination of the Concentration of Micafungin in Human Plasma by HPLC and its Application in Critical Patients
- Vol. 20, Issue 9, Pages: 1090-1092(2020)
- 作者机构：
  
  中国医科大学附属盛京医院药学部
- 作者简介：
- 基金信息：
- DOI：10.14009/j.issn.1672-2124.2020.09.017
  CLC： R969.1
- Published：2020，
扫描看全文
[1]赵明明,陈亚南,路童,李国飞,肇丽梅.高效液相色谱法测定人血浆中米卡芬净浓度及其在重症患者中的应用[J].中国医院用药评价与分析,2020,20(09):1090-1092.

ZHAO Mingming, CHEN Yanan, LU Tong, et al. Determination of the Concentration of Micafungin in Human Plasma by HPLC and its Application in Critical Patients. [J]. 20(9):1090-1092(2020)
[1]赵明明,陈亚南,路童,李国飞,肇丽梅.高效液相色谱法测定人血浆中米卡芬净浓度及其在重症患者中的应用[J].中国医院用药评价与分析,2020,20(09):1090-1092. DOI： 10.14009/j.issn.1672-2124.2020.09.017.

ZHAO Mingming, CHEN Yanan, LU Tong, et al. Determination of the Concentration of Micafungin in Human Plasma by HPLC and its Application in Critical Patients. [J]. 20(9):1090-1092(2020) DOI： 10.14009/j.issn.1672-2124.2020.09.017.

摘要

目的:建立测定人血浆中米卡芬净浓度的高效液相色谱法。方法:以氯唑沙宗为内标

血浆样品用乙腈沉淀蛋白处理后

经C

色谱柱进行分离

流动相为乙腈-5 mmol/L醋酸铵（V∶V=40∶60）

流速为1.0 ml/min

柱温为35℃

检测波长为273 nm

同时进行方法学的验证

并将其应用于重症患者血浆中米卡芬净浓度的测定。结果:米卡芬净在0.2～25.0μg/ml浓度范围内线性关系良好

最低定量下限为0.2μg/ml

低、中及高浓度质量控制样品的回收率分别为88.54%、83.27%及88.16%

日内和日间精密度均在±15%之内。结论:本方法简单快速、准确可靠

可用于临床重症患者米卡芬净血药浓度的监测。

Abstract

OBJECTIVE: To establish a HPLC method for determining the concentration of micafungin in human plasma. METHODS: Chlorzoxazone was set as internal standard substance

the plasma sample was proceeded by acetonitrile protein precipitation and isolated by C

chromatographic column

with the mobile phase was acetonitrile-5 mmol/L of ammonium acetate （V ∶V = 40 ∶60）

the flow rate was 1. 0 ml/min

the column temperature was 35 ℃ and the detecting wavelength was 273 nm

methodological verification was conducted and applied to determine the concentration of micafungin in human plasma in critical patients. RESULTS: Micafungin was in a good linear relationship in the concentration range of 0. 2-25. 0 μg/ml

with the lowest quantification limit was 0. 2 μg/ml. The recovery rates of low

medium and high concentration quality control samples were respectively 88. 54%

83. 27% and 88. 16%

the intra-day and inter-day precision were both within ±15%. CONCLUSIONS: This method is simple

fast

accurate and reliable

which can be used to monitor the blood concentration of micafungin in critical patients.

关键词

米卡芬净高效液相色谱法血药浓度

Keywords

MicafunginHigh-performance liquid chromatographyBlood concentration

references

查看原文

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Pharmacodynamic Analysis of Levetiracetam in the Treatment of Epilepsy in Children

Pharmacoeconomic Evaluation of Caspofungin and Micafungin in the Treatment of Invasive Candidiasis

Retrospective Analysis of Blood Concentration Monitoring of Cyclosporine A in the Treatment of Psoriasis

Research on the Value of Blood Concentration and Gene Detection of Methotrexate in the Treatment of Low-Risk Gestational Trophoblastic Neoplasia

Effect of Configuration Concentration and Patient’s Basic Renal Function on the Safety of Vancomycin

Related Author

LI Xingang

LU Wei

WEI Minji

WU Ye

WANG Li

WANG Yinghui

CUI Xiangli

HAN Baofeng

Related Institution

Peking University School of Pharmaceutical Sciences

Dept.of Pediatric, Peking University First Hospital

Dept.of Pediatric, Beijing Friendship Hospital, Capital Medical University

Dept.of Pharmacy, Xiyuan Hospital, China Academy of Chinese Medical Sciences

College of Pharmacy, Capital Medical University

⁰