Establishment of Reversed Phase High Performance Liquid Chromatography Method for Plasma Teicoplanin Concentration

YANG Yunyun; CAI Lu; WANG Xuebin; GAO Lihong; ZHANG Lingpeng; ZHANG Wenjing; WANG Zhuo

doi:10.14009/j.issn.1672-2124.2020.09.004

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Establishment of Reversed Phase High Performance Liquid Chromatography Method for Plasma Teicoplanin Concentration

更新时间：2022-10-08

- Establishment of Reversed Phase High Performance Liquid Chromatography Method for Plasma Teicoplanin Concentration
- Vol. 20, Issue 9, Pages: 1041-1043(2020)
- 作者机构：
  
  上海长海医院药学部
- 作者简介：
- 基金信息：
- DOI：10.14009/j.issn.1672-2124.2020.09.004
  CLC： R978.1
- Published：2020，
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[1]杨云云,蔡璐,王学彬,高丽红,张凌鹏,张文静,王卓.血浆替考拉宁浓度的反相高效液相色谱分析方法的建立[J].中国医院用药评价与分析,2020,20(09):1041-1043+1048.

YANG Yunyun, CAI Lu, WANG Xuebin, et al. Establishment of Reversed Phase High Performance Liquid Chromatography Method for Plasma Teicoplanin Concentration. [J]. 20(9):1041-1043(2020)
[1]杨云云,蔡璐,王学彬,高丽红,张凌鹏,张文静,王卓.血浆替考拉宁浓度的反相高效液相色谱分析方法的建立[J].中国医院用药评价与分析,2020,20(09):1041-1043+1048. DOI： 10.14009/j.issn.1672-2124.2020.09.004.

YANG Yunyun, CAI Lu, WANG Xuebin, et al. Establishment of Reversed Phase High Performance Liquid Chromatography Method for Plasma Teicoplanin Concentration. [J]. 20(9):1041-1043(2020) DOI： 10.14009/j.issn.1672-2124.2020.09.004.

摘要

目的:建立反相高效液相色谱法（reversed phase high performance liquid chromatography

RP-HPLC）测定人血浆中替考拉宁的浓度。方法:采用Waters C

色谱柱（150 mm×4.6 mm

5μm）

流动相为0.02 mol/L的NaH

（pH=2.50）-乙腈（V∶V=74.5∶25.5）

内标物为对羟基苯甲酸甲酯

流速为1.0 ml/min

检测波长为240 nm

进样体积为0.020 ml

柱温为35℃。结果:在上述色谱条件下

替考拉宁和内标的分离度均良好（R

1.5）

替考拉宁保留时间约9 min

内标物保留时间约为11 min

替考拉宁在3.125～100μg/ml（r=99.96%

n=6）范围内线性关系良好;定量下限为3.125μg/ml时

信噪比（S/N）=18.5

批内和批间的准确度和精密度均符合要求。结论:本研究方法适合测定人血浆中替考拉宁的浓度

为替考拉宁的个体化用药提供指导。

Abstract

OBJECTIVE: To establish a method of reversed phase high performance liquid chromatography （RPHPLC） for the determination of teicoplanin in human plasma. METHODS: Waters C

column （150 mm × 4. 6 mm

5 μm） was used

the mobile phase was 0. 02 mol/L of NaH

（pH = 2. 50）-acetonitrile （V ∶V = 74. 5 ∶25. 5）

the internal standard substance was methyl parahydroxybenzoats

with the flow rate was 1. 0 ml/min

the detection wavelength was 240 nm

the injection volume was 0. 020 ml and the column temperature was 35 ℃ . RESULTS: Under the above chromatographic conditions

both the resolutions of teicoplanin and internal standard substance were good （R

1. 5）

the retention time of teicoplanin was about 9 min and the retention time of internal standard substance was about 11 min

the linear relationship was good in the range of 3. 125-100 μg/ml （r = 99. 96%

n = 6）; when the lower limit of quantification was 3. 125 μg/ml

the signal-to-noise ratio （S/N） was 18. 5

both the accuracy and precision of intra-batch and inter-batch meet the requirements. CONCLUSIONS: This research method is suitable for determining the concentration of teicoplanin in human plasma and provides guidance for the individualized medication of teicoplanin.

关键词

替考拉宁反相高效液相色谱法血药浓度

Keywords

TeicoplaninRP-HPLCBlood concentration

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