OBJECTIVE: To probe into the effect of dihydrotanshinoneⅠon biological behavior of ovarian cancer cell based on nuclear factor kappa B（ NF-κB） signaling pathway. METHODS: Tetrazolium colorimetry

cell scratch test

flow cytometry and Western blot were adopted to detect the effect of different concentrations of dihydrotanshinone Ⅰ on the proliferation

migration and apoptosis of ovarian cancer cell strain SKOV3 and the protein expression of NF-κB signaling pathway. RESULTS: Compared with the concentration of 0 μmol/L

the optical density（ OD） of SKOV3 cell was significantly lower under the processing of 2

4

6

8 and 10 μmol/L dihydrotanshinoneⅠ

with significantly longer scratch length and higher apoptosis rate; compared with the application of dihydrotanshinoneⅠ for 24 h

the OD of SKOV3 cell was significantly lower after 48 h

with significantly longer scratch length and higher apoptosis rate

with statistically significant differences（ P<0. 05）; with the increase of the concentration of dihydrotanshinone Ⅰ

the inhibition of proliferation and migration of SKOV3 cells by dihydrotanshinoneⅠand the promotion of apoptosis of SKOV3 cells are more obvious

which are dose-dependent and time-dependent. The comparison of relative expression among NF-κB

P65 and B-cell lymphoma-2（ Bcl-2） protein of SKOV3 cells after the processing of different concentrations of dihydrotanshinone Ⅰwere statistically significant（ P<0. 05）; with the increase of concentration of dihydrotanshinoneⅠ

it had more obvious inhibiting effect on the expression of NF-κB

P65 and Bcl-2 protein of SKOV3 cells

with dose-dependent. CONCLUSIONS: DihydrotanshinoneⅠmay inhibit the proliferation and migration of human ovarian cancer cell line SKOV3 by down regulating the expression of NF-κB

P65 and Bcl-2 protein

inhibiting the NF-κB signaling pathway and inducing apoptosis.