Research on the Mechanism of “Dandelion-Selfheal”in Treatment of Breast Cancer Based on Network Pharmacology

TANG Jiwei; ZHENG San; CAO Xueshuai; ZHANG Baiyin; GU Hui; GUO Yijie

doi:10.14009/j.issn.1672-2124.2020.01.011

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Research on the Mechanism of “Dandelion-Selfheal”in Treatment of Breast Cancer Based on Network Pharmacology

更新时间：2022-08-15

- Research on the Mechanism of “Dandelion-Selfheal”in Treatment of Breast Cancer Based on Network Pharmacology
- Vol. 20, Issue 1, Pages: 44-49(2020)
- 作者机构：
  
  1. 湖南省第二人民医院麻醉手术科
  2. 湖南中医药大学药学院
- 作者简介：
- 基金信息：
- DOI：10.14009/j.issn.1672-2124.2020.01.011
  CLC： R285
- Published：2020，
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[1]唐吉伟,郑叁,曹学帅,张柏银,顾晖,郭亦杰.基于网络药理学的“蒲公英-夏枯草”药对治疗乳腺癌作用机制研究[J].中国医院用药评价与分析,2020,20(01):44-49.

TANG Jiwei, ZHENG San, CAO Xueshuai, et al. Research on the Mechanism of “Dandelion-Selfheal”in Treatment of Breast Cancer Based on Network Pharmacology. [J]. 20(1):44-49(2020)
[1]唐吉伟,郑叁,曹学帅,张柏银,顾晖,郭亦杰.基于网络药理学的“蒲公英-夏枯草”药对治疗乳腺癌作用机制研究[J].中国医院用药评价与分析,2020,20(01):44-49. DOI： 10.14009/j.issn.1672-2124.2020.01.011.

TANG Jiwei, ZHENG San, CAO Xueshuai, et al. Research on the Mechanism of “Dandelion-Selfheal”in Treatment of Breast Cancer Based on Network Pharmacology. [J]. 20(1):44-49(2020) DOI： 10.14009/j.issn.1672-2124.2020.01.011.

摘要

目的:基于网络药理学方法

探讨"蒲公英-夏枯草"药对治疗乳腺癌作用机制。方法:利用中药系统药理学数据库和分析平台（traditional Chinese medicine systems pharmacology database and analysis platform

TCMSP）筛选出"蒲公英-夏枯草"药对的主要化学成分及作用靶点

建立"成分-靶点"蛋白质-蛋白质相互作用（protein-protein interaction

PPI）网络。利用比较毒理基因组学数据库（comparative toxicogenomics database

CTD）检索与乳腺癌相关的基因

采用Cytoscape 3. 6. 1软件构建"疾病-靶点"PPI网络。基于Cytoscape 3. 6. 1软件的Merge功能

合并"成分-靶点"和"疾病-靶点"的PPI网络

筛选出主要核心网络

借助David数据库、Reactome数据库及Omicshare平台

进行基因本体（gene ontology

GO）富集分析、京都基因与基因组百科全书（Kyoto encyclopedia of genes and genomes

KEGG）通路富集分析。通过动物实验

验证"蒲公英-夏枯草"药对的抗肿瘤作用机制。结果:筛选得到含383个节点、594条连接的"蒲公英-夏枯草"药对"成分-靶点"PPI网络

含500个靶点的乳腺癌"疾病-靶点"PPI网络。利用网络合并

最终建立了含61个核心靶点的"成分-靶点-疾病"网络。基因GO功能分析结果主要与生物过程和分子功能有关;基因KEGG通路富集分析结果与免疫系统、性激素分泌以及肿瘤细胞增殖与凋亡有关。动物实验结果显示

"蒲公英-夏枯草"药对能提高乳腺癌模型小鼠白细胞介素6水平

抑制乳腺癌细胞增殖

促进乳腺癌细胞凋亡。结论:"蒲公英-夏枯草"药对治疗乳腺癌的可能机制与调节免疫系统、性激素分泌以及肿瘤细胞增殖与凋亡有关。

Abstract

OBJECTIVE: To probe into the mechanism of "dandelion-selfheal"in treatment of breast cancer based on network pharmacology. METHODS: To screen the main chemical component and target spot of the herbal pair of "dandelion-selfheal"through the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform（ TCMSP）

so as to establish the protein-protein interaction（ PPI） network of "component-target". Comparative toxicogenomics database（ CTD） was used to retrieve genes related to breast cancer

Cytoscape 3. 6. 1 was adopted to construct "disease-target " PPI network. Based on the Merge function of Cytoscape 3. 6. 1

the PPI networks of "component-target"and "disease-target"were merged to screen out the main core network. With the help of the David database

Reactome database and Omicshare platform

gene ontology（ GO） enrichment analysis and Kyoto encyclopedia of genes and genomes（ KEGG） pathway enrichment analysis were conducted. Through animal experiments

the anti-tumor mechanism of the herbal pair of "dandelion-selfheal"was verified. RESULTS: A PPI network of "component-target"containing 383 nodes and 594 linked herbal pair of "dandelion-selfheal"and a PPI network of "disease-target"of breast cancer containing 500 target spots were screened out. With utilization of the network merger

a "component-target-disease"network containing 61 core targets was finally established. The result of gene GO function analysis was mainly related to biological process and molecular function; the result of gene KEGG pathway enrichment analysis was related to immune system

sex hormone secretion

proliferation and apoptosis of tumor cell. The result of animal experiment showed that the herbal pair of "dandelion-selfheal"can increase the IL-6 level

inhibit the proliferation of breast cancer cells and promote the apoptosis of breast cancer cells in model mice.CONCLUSIONS: The possible mechanism of herbal pair of"dandelion-selfheal"in treatment of breast cancer is related to immune system regulation

sex hormone secretion

tumor cell proliferation and apoptosis.

关键词

蒲公英夏枯草乳腺癌网络药理学

Keywords

DandelionSelfhealBreast cancerNetwork pharmacology

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