OBJECTIVE: To primarily explore the mechanism of motherwort injection for hemostasis and regulating menstruation based on network pharmacology. METHODS: Traditional Chinese medicine systems pharmacology database and analysis platform（ TCMSP） and STITCH database were retrieved for the action targets of stachydrine

choline

trigonelline in motherwort injection. The therapeutic target database（ TTD） and Genetic pharmacology and pharmacogenomics database（ PharmGKB） were retrieved for the action targets of bleeding and irregular menstruation disease. Cytoscape 3. 6. 1 software was used to established the Compound-Compound Target network

Target-Disease network

Protein-Protein Interaction network and Drug-Compound-Target-Pathway network. Gene ontology（ GO）functional enrichment analysis and Kyoto encyclopedia of genes and genomes（ KEGG） pathway enrichment analysis were carried out with the help of DAVID v6. 7 platform to study the mechanism of motherwort injection for hemostasis and regulating menstruation. RESULTS: The active ingredients of motherwort injection could act on 9 potential targets

including PTGS1

PTGS2

CYP2 C9

CYP2 C19 and CYP2 D6. These targets had molecular functions such as binding with heme

binding with oxygen

binding with iron and regulating enzyme activity

and can be used in the treatment of postpartum hemorrhage

abnormal uterine bleeding

irregular menstruation and other gynecological diseases. There were 5 pathways

mainly included serotonin-containing synaptic pathway

drug metabolism-hepatic drug enzyme CYP pathway and linoleic acid metabolism pathway. CONCLUSIONS: This study preliminarily predict the molecular mechanism of motherwort injection for hemostasis and regulating menstruation

and provide the idea and clue for further revealing the mechanism of action on motherwort injection.