Relief of ODN2088 for Acute Lung Injury in Rats with Sepsis Induced by Mitochondrial DNA

Tang Tian; Li Shaojun; Guo Pengfei; Yang Lin; Ao Xiaoxiao; Hu Lan; Tan Liping

doi:10.13407/j.cnki.jpp.1672.108X.2021.03.001

您当前的位置：

首页 >

文章列表页 >

Relief of ODN2088 for Acute Lung Injury in Rats with Sepsis Induced by Mitochondrial DNA

更新时间：2022-11-03

- Relief of ODN2088 for Acute Lung Injury in Rats with Sepsis Induced by Mitochondrial DNA
- Vol. 27, Issue 3, Pages: 1-6(2021)
- 作者机构：
  
  重庆医科大学附属儿童医院，儿童发育疾病研究教育部重点实验室，国家儿童健康与疾病临床医学研究中心，儿童发育重大疾病国家国际科技合作基地，儿科学重庆市重点实验室,重庆,400014
- 作者简介：
- 基金信息：
- DOI：10.13407/j.cnki.jpp.1672.108X.2021.03.001
  CLC：
- Published：2021，
扫描看全文
Tang Tian, Li Shaojun, Guo Pengfei, et al. Relief of ODN2088 for Acute Lung Injury in Rats with Sepsis Induced by Mitochondrial DNA. [J]. 27(3):1-6(2021)
DOI：

Tang Tian, Li Shaojun, Guo Pengfei, et al. Relief of ODN2088 for Acute Lung Injury in Rats with Sepsis Induced by Mitochondrial DNA. [J]. 27(3):1-6(2021) DOI： 10.13407/j.cnki.jpp.1672.108X.2021.03.001.

摘要

目的：探讨血浆线粒体DNA（mitochondrial DNA，mtDNA）含量与脓毒症大鼠急性肺损伤（acute lung injury，ALI）程度的关系及选择性TLR9受体抑制剂ODN2088对脓毒症大鼠ALI的保护作用。方法：按随机数字表法将80只大鼠分为假手术（sham）组、脓毒症（sepsis）组、脓毒症大鼠腹腔注射mtDNA 1.4 mg/kg（sepsis+mtDNA）组、脓毒症大鼠腹腔注射mtDNA 1.4 mg/kg+ODN2088 1 mg/kg（sepsis+mtDNA+ODN）组，每组各20只。采用盲肠结扎穿孔（CLP）制备脓毒症合并ALI模型。术后6 h、12 h采集血液标本、肺泡灌洗液（bronchoalveolar lavage fluid，BALF）和肺组织以检测血浆mtDNA及肺组织TLR9/p38MAPK表达水平。结果：与sham组比较，sepsis组大鼠病死率高，血浆mtDNA水平和肺组织TLR9/p38MAPK表达增加，ALI程度加重[肺损伤评分、BALF中炎症细胞、肺（湿-干）/干重比和基因表达增加，P均<0.05）]。与sepsis组大鼠相比，sepsis+mtDNA组大鼠的病死率、血浆mtDNA水平、TLR9/p38MAPK表达增加，ALI程度加重（P均<0.05）。sepsis+mtDNA+ODN组大鼠较sepsis+mtDNA组大鼠病死率、血浆mtDNA水平、TLR9/p38MAPK表达及ALI程度降低（P均<0.05）。结论：血浆mtDNA水平与脓毒症所致ALI有关，TLR9抑制剂ODN 2088可减轻mtDNA诱导的脓毒症大鼠ALI。

Abstract

Objective: To probe into the correlation between plasma mitochondrial DNA (mtDNA) content and severity of acute lung injury (ALI) in rats with sepsis and the protective effects of selective Toll-like receptor 9 (TLR9) inhibitor ODN2088 on ALI in rats with sepsis. Methods: According to the random number table method

80 rats were divided into the sham surgery (sham) group

sepsis (sepsis) group

intraperitoneal injection of mtDNA 1.4 mg/kg (sepsis+mtDNA) group and intraperitoneal injection of mtDNA 1.4 mg/kg+ODN2088 1 mg/kg (sepsis+mtDNA+ODN) group

with 20 rats in each group. The model of sepsis complicated with ALI was established by cecal ligation and perforation (CLP). After surgery of 6 and 12 h

blood samples

bronchoalveolar lavage fluid (BALF) and lung tissues were collected to detect plasma mtDNA and TLR9/p38 mitogen activated protein kinase (MAPK) expression levels in lung tissues. Results: Compared with the sham group

the sepsis group had higher mortality

increased levels of plasma mtDNA and TLR9/p38 MAPK expression in lung tissues

and increased ALI degree [lung injury score

inflammatory cells in BALF

lung (wet-dry)/dry weight ratio and gene expression

P<0.05]. Compared with the sepsis group

the mortality rate

plasma mtDNA

TLR9/p38MAPK expression and ALI degree increased in the sepsis+mtDNA group (P<0.05). Compared with the sepsis+mtDNA+ODN group

the mortality rate

plasma mtDNA

TLR9/p38MAPK expression and ALI degree decreased in the sepsis+mtDNA+ODN group (P<0.05). Conclusion: Plasma mtDNA is correlated with ALI induced by sepsis

and TLR9 inhibitor ODN2088 can relieve ALI in rats with sepsis induced by mtDNA.

关键词

脓毒症急性肺损伤mtDNATLR9/p38受体

Keywords

sepsisacute lung injurymitochondrial DNATLR9/p38 receptor

references

查看原文

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Narciclasine-Activated AKT/mTOR Pathway in Alleviating Hyperoxia-Induced Acute Lung Injury in Neonatal Rats

Risk Factors for Prognosis of Sepsis Complicated with Acute Kidney Injury in Children

Related Author

Han Xu

Zhu Ping

Liu Ni

Wu Zongyue

Yang Jing

Wang Lili

Zhao Zhongxiu

Zhang Xinqing

Related Institution

Nanyang Central Hospital

Xuzhou Children’s Hospital

⁰