Determination of Blood Concentration of Fluconazole in Neonates by High Performance Liquid Chromatography

Qin Donjie; Liang Jing; Wei Yanfei; Huang Yuling; Li Ping; Jiang Bei; Zhuge Mingli; Xu Feng; Wang Honghong

doi:10.13407/j.cnki.jpp.1672.108X.2020.12.012

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Determination of Blood Concentration of Fluconazole in Neonates by High Performance Liquid Chromatography

更新时间：2022-11-03

- Determination of Blood Concentration of Fluconazole in Neonates by High Performance Liquid Chromatography
- Vol. 26, Issue 12, Pages: 40-43(2020)
- 作者机构：
  
  1. 3,4,5,6,7(1. 柳州市质量检验检测研究中心,广西,柳州,545006
  2. 柳州市妇幼保健院,广西,柳州,545001
  3. 广西科技大学附属妇产医院、儿童医院,广西,柳州,545001
  4. 广西医科大学公共卫生学院,广西,南宁,530021
  5. 广西基因组与个体化医学重点实验室,广西,南宁,530021
  6. 广西基因组与个体化医学协同创新中心,广西,南宁,530021
  7. 广西高等学校重点实验室,广西,南宁,530021
- 作者简介：
- 基金信息：
- DOI：10.13407/j.cnki.jpp.1672.108X.2020.12.012
  CLC：
- Published：2020，
  
  Published Online：10 December 2020，
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Qin Donjie, Liang Jing, Wei Yanfei, et al. Determination of Blood Concentration of Fluconazole in Neonates by High Performance Liquid Chromatography. [J]. 26(12):40-43(2020)
DOI：

Qin Donjie, Liang Jing, Wei Yanfei, et al. Determination of Blood Concentration of Fluconazole in Neonates by High Performance Liquid Chromatography. [J]. 26(12):40-43(2020) DOI： 10.13407/j.cnki.jpp.1672.108X.2020.12.012.

摘要

目的：建立测定新生儿体内氟康唑血药浓度的高效液相色谱（HPLC）法。方法：HPLC色谱条件为色谱柱Diamonsil C18柱（5 μm，4.6mm×250 mm）；流动相甲醇﹕磷酸盐缓冲液（pH 7.0）=45︰55；检测波长261 nm；流速1 mL/min；柱温30℃；进样量10 μL。供试品溶液的制备，取样品加入内标后用乙酸乙酯萃取，有机相用氮气吹干，以甲醇溶解，进样测定。结果：该方法氟康唑在1.0~50.0 μg/mL范围内有良好线性关系，精密度、稳定性及回收率较好。结论：该方法操作简单、重复性好、准确可靠，可作为新生儿体内预防用氟康唑HPLC含量测定的方法。

Abstract

Objective: To establish the high performance liquid chromatography (HPLC) method for determination of blood concentration of fluconazole in neonates. Methods: HPLC was carried out on Diamonsil C18 column (5 μm

4.6 mm×250 mm). The mobile phase consisted of methanol and phosphate buffer solution (pH 7.0)=45︰55 at the flow of 1.0 mL/min

and the detection wavelength was 261 nm. Column temperature was 30 ℃ and sample amount was 10 μL. The blood sample with internal standard was extracted with ethyl acetate

the organic extract was concentrated to dryness under nitrogen

and the residue was dissolved in methanol and analyzed. Results: The standard curve was good linear from 1.0 to 50.0 μg/mL

and the precision

stability and recovery rate were good. Conclusion: This method is simple

repeatable

accurate and reliable

and can be used for determination of blood concentration of fluconazole in neonates by HPLC.

关键词

新生儿氟康唑含量测定高效液相色谱

Keywords

neonatesfluconazoleeterminationhigh performance liquid chromatography

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