Determination of Caffeine Blood Concentration in Neonates by High Performance Liquid Chromatography and Its Clinical Application

Li Sai; Li Shoulin; Su Lingying

doi:10.13407/j.cnki.jpp.1672-108X.2023.08.009

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Determination of Caffeine Blood Concentration in Neonates by High Performance Liquid Chromatography and Its Clinical Application

更新时间：2023-08-23

- Determination of Caffeine Blood Concentration in Neonates by High Performance Liquid Chromatography and Its Clinical Application
- Vol. 29, Issue 8, Pages: 31-34(2023)
- 作者机构：
  
  1. 淮安市妇幼保健院,江苏,淮安,223001
  2. 南京医科大学附属淮安第一人民医院,江苏,淮安,223300
- 作者简介：
- 基金信息：
- DOI：10.13407/j.cnki.jpp.1672-108X.2023.08.009
  CLC：
- Published：2023，
  
  Published Online：10 August 2023，
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Li Sai, Li Shoulin, Su Lingying. Determination of Caffeine Blood Concentration in Neonates by High Performance Liquid Chromatography and Its Clinical Application. [J]. 29(8):31-34(2023)
DOI：

Li Sai, Li Shoulin, Su Lingying. Determination of Caffeine Blood Concentration in Neonates by High Performance Liquid Chromatography and Its Clinical Application. [J]. 29(8):31-34(2023) DOI： 10.13407/j.cnki.jpp.1672-108X.2023.08.009.

摘要

目的:建立测定新生儿血清中咖啡因血药浓度的高效液相色谱法

并对使用咖啡因治疗原发性呼吸暂停新生儿的血药浓度进行测定。方法:色谱柱为 ZORBAX Extend C18 柱

流动相为乙腈-水(19 ∶ 81

V/ V)

柱温 25 ℃

流速 1 mL/ min

检测波长273 nm。结果:咖啡因血药浓度在 1. 0~160. 0 μg / mL 范围内呈良好的线性关系(r = 0. 998 9)

平均回收率 98. 40% ~ 102. 40%

日内与日间相对标准差(RSD)≤9. 63%

检测 10 例患儿给药 7 d 后咖啡因血药浓度为(20. 53±11. 12)μg / mL。结论:建立的高效液相色谱法准确、快速

经验证适用于新生儿血清中咖啡因血药浓度测定。

Abstract

Objective: To establish the high performance liquid chromatography (HPLC) method for the determination of caffeine blood concentration in neonates

and to determine the concentration of caffeine in neonates with primary apnea. Methods: The determination was performed on ZORBAX Extend C18 column with mobile phase acetonitrile-water (19 ∶ 81

V/ V) at the column temperature of 25 ℃ and flow rate of 1 mL/ min. The detection wavelength was 273 nm. Results: Caffeine blood concentration showed good linearity ( r =0. 998

9) in the range of 1. 0 to 160. 0 μg / mL

with mean recoveries of 98. 40% to 102. 40%. Inter-day and intra-day relative standard deviation (RSD) was ≤9. 63%. The average caffeine blood concentration in 10 children was ( 20. 53± 11. 12) μg / mL after 7 d ofadministration. Conclusion: The established high performance liquid chromatography method is accurate

rapid and validated for the determination of caffeine blood concentration in neonates.

关键词

新生儿咖啡因高效液相色谱法血药浓度

Keywords

neonatescaffeinehigh performance liquid chromatographyblood concentration

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