Establishment of Sirolimus Blood Concentration Monitoring Method and Its Application in Children with Hemangioma

Lin Zhiyan; Huang Xiaohui; Yang Ping

doi:10.13407/j.cnki.jpp.1672-108X.2022.11.004

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Establishment of Sirolimus Blood Concentration Monitoring Method and Its Application in Children with Hemangioma

更新时间：2023-04-14

- Establishment of Sirolimus Blood Concentration Monitoring Method and Its Application in Children with Hemangioma
- Vol. 28, Issue 11, Pages: 14-18(2022)
- 作者机构：
  
  上海交通大学医学院附属新华医院,上海,200092
- 作者简介：
- 基金信息：
- DOI：10.13407/j.cnki.jpp.1672-108X.2022.11.004
  CLC：
- Published：2022，
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Lin Zhiyan, Huang Xiaohui, Yang Ping. Establishment of Sirolimus Blood Concentration Monitoring Method and Its Application in Children with Hemangioma. [J]. 28(11):14-18(2022)
DOI：

Lin Zhiyan, Huang Xiaohui, Yang Ping. Establishment of Sirolimus Blood Concentration Monitoring Method and Its Application in Children with Hemangioma. [J]. 28(11):14-18(2022) DOI： 10.13407/j.cnki.jpp.1672-108X.2022.11.004.

摘要

目的：建立测定人全血中西罗莫司浓度的超高压液相色谱-串联质谱（UPLC-MS/MS）方法。方法：色谱柱为Agilent SB-C18（2.1 mm×50 mm，1.8 μm），流动相为水（A）-乙腈（0.1%甲酸，B），流速0.3 mL/min，梯度洗脱。依维莫司为内标，电喷雾离子源，多反应离子监测模式，正离子模式检测。西罗莫司和依维莫司离子对分别为m/z 936.5/409.5、980.6/389.3。同时采用该方法对我院血管瘤患儿西罗莫司血药浓度进行监测。结果：西罗莫司在0.625~40.000 ng/mL浓度范围内相对峰面积线性关系良好（R2=0.999 5），日内、日间RSD均小于10%，提取回收率91.95%~118.50%，基质效应81.67%~107.84%。结论：本方法灵敏、准确、快速，能满足临床常规监测西罗莫司血药浓度的需求

Abstract

Objective: To establish the ultra high pressure liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method for determination of sirolimus in human whole blood. Methods: The analysis was carried out on the Agilent SB-C18 (2.1 mm×50 mm

1.8 μm) column with gradient elution. The mobile phase was water (A)-acetonitrile (0.1% formic acid

B) at a flow rate of 0.3 mL/min. Everolimus was used as internal standard. The determination was performed on a triple quadrupole tandem mass spectrometer by multiple reactions monitoring mode via electrospray ionization source. The quantitative ion transition m/z of sirolimus and everolimus were 936.5/409.5 and 980.6/389.3. Meanwhile

the blood concentration of sirolimus in children with hemangioma was monitored by this method. Results: Sirolimus showed good linearity (r=0.999

7) in relative peak area over the concentration range of 0.625 to 40.000 ng/mL with intra-day and inter-day RSD less than 10%

extraction recoveries from 91.95% to 118.50% and matrix effects from 81.67% to 107.84%. Conclusion: The method is sensitive

accurate and rapid for monitoring of whole blood concentration of sirolimus

which can meet the needs of routine clinical monitoring of sirolimus blood concentration

关键词

西罗莫司超高压液相色谱-串联质谱血管瘤儿童治疗药物浓度监测

Keywords

sirolimusultra high pressure liquid chromatography-tandem mass spectrometryhemangiomachildrentherapeutic drug monitoring

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