Determination of 8 Main Components in Xiao’er Qingre Huatan Suppository by High Performance Liquid Chromatography-Quantitative Analysis of Multi-Components by Single-Marker

Zhang Chuanxiang; He Miao; Ma Danfeng; Wang Haibin

doi:10.13407/j.cnki.jpp.1672-108X.2022.10.008

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Determination of 8 Main Components in Xiao’er Qingre Huatan Suppository by High Performance Liquid Chromatography-Quantitative Analysis of Multi-Components by Single-Marker

更新时间：2022-11-04

- Determination of 8 Main Components in Xiao’er Qingre Huatan Suppository by High Performance Liquid Chromatography-Quantitative Analysis of Multi-Components by Single-Marker
- Vol. 28, Issue 10, Pages: 26-30(2022)
- 作者机构：
  
  1. 湖南省儿童医院,长沙,410007
  2. 湖南省药品检验研究院,长沙,410001
- 作者简介：
- 基金信息：
- DOI：10.13407/j.cnki.jpp.1672-108X.2022.10.008
  CLC：
- Published：2022，
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Zhang Chuanxiang, He Miao, Ma Danfeng, et al. Determination of 8 Main Components in Xiao’er Qingre Huatan Suppository by High Performance Liquid Chromatography-Quantitative Analysis of Multi-Components by Single-Marker. [J]. 28(10):26-30(2022)
DOI：

Zhang Chuanxiang, He Miao, Ma Danfeng, et al. Determination of 8 Main Components in Xiao’er Qingre Huatan Suppository by High Performance Liquid Chromatography-Quantitative Analysis of Multi-Components by Single-Marker. [J]. 28(10):26-30(2022) DOI： 10.13407/j.cnki.jpp.1672-108X.2022.10.008.

摘要

目的：采用高效液相一测多评（HPLC-QAMS）法，建立小儿清热化痰栓中马钱子苷、当药苷、断氧化马钱子苷、甘草苷、甘草酸、连翘酯苷B、连翘酯苷A和连翘苷含量的检测方法。方法：采用Agilent Zorbax SB-C18色谱柱，以乙腈-0.2%磷酸水溶液为流动相，梯度洗脱；检测波长分别为237 nm（检测马钱子苷、当药苷、断氧化马钱子苷、甘草苷和甘草酸）和275 nm（检测连翘酯苷B、连翘酯苷A和连翘苷）；流速1.0 mL/min；柱温30 ℃。以甘草苷为内参物，通过测定其与马钱子苷、当药苷、断氧化马钱子苷、甘草酸、连翘酯苷B、连翘酯苷A和连翘苷的相对校正因子（RCF），计算各成分含量，同时与外标法（ESM）实测值进行对比，验证HPLC-QAMS的可行性。结果：马钱子苷、当药苷、断氧化马钱子苷、甘草苷、甘草酸、连翘酯苷B、连翘酯苷A和连翘苷质量浓度分别在4.08~102.00、0.77~19.25、4.59~114.75、1.35~33.75、2.74~68.50、1.61~40.25、7.73~193.25、2.19~54.75 μg/mL范围内与峰面积线性关系良好（r≥0.999 1），平均加样回收率及RSD分别为99.45%（0.96%）、97.49%（1.45%）、98.85%（1.08%）、99.13%（0.78%）、100.04%（0.76%）、98.63%（1.59%）、100.07%（0.60%）和96.99%（1.26%）。小儿清热化痰栓中各成分HPLC-QAMS计算含量值与外标法实测含量值无明显差异。结论：所建立的HPLC-QAMS操作简便易行，成本较低，可用于小儿清热化痰栓多指标成分的质量评价。

Abstract

Objective: To establish the determination method of loganin

sweroside

secoxyloganin

liquiritin

glycyrrhizic acid

forsythoside B

forsythoside A and forsythin in Xiao’er Qingre Huatan suppository based on high performance liquid chromatography-quantitative analysis of multi-components by single-marker (HPLC-QAMS) method. Methods: Agilent Zorbax SB-C18 was adopted

acetonitrile-0.2% phosphoric acid solution was the mobile phase by gradient elution. The detection wavelength was set at 237 nm for loganin

sweroside

secoxyloganin

liquiritin and glycyrrhizic acid

and 275 nm for forsythoside B

forsythoside A and forsythin. The flow rate was 1.0 mL/min at column temperature of 30 ℃. Liquiritin was used as an internal standard

the relative correction factors (RCF) of loganin

sweroside

secoxyloganin

glycyrrhizic acid

forsythoside B

forsythoside A and forsythin were calculated. The method was validated by comparison of the quantitative results between external standard method (ESM) and HPLC-QAMS method. Results: Loganin

sweroside

secoxyloganin

liquiritin

glycyrrhizic acid

forsythoside B

forsythoside A and forsythin showed good linear relationships within the ranges from 4.08 to 102.00

from 0.77 to 19.25

from 4.59 to 114.75

from 1.35 to 33.75

from 2.74 to 68.50

from 1.61 to 40.25

from 7.73 to 193.25 and from 2.19 to 54.75 μg/mL (r≥0.999

the average recoveries and RSD were respectively 99.45% (0.96%)

97.49% (1.45%)

98.85% (1.08%)

99.13% (0.78%)

100.04% (0.76%)

98.63% (1.59%)

100.07% (0.60%) and 96.99% (1.26%). There was no significant difference between the contents calculated by HPLC-QAMS and measured by external standard method for each component in Xiao’er Qingre Huatan suppository. Conclusion: The HPLC-QAMS method is simple with lower cost

and can be used for the multi-index component quality evaluation of Xiao’er Qingre Huatan suppository.

关键词

小儿清热化痰栓高效液相一测多评法多指标成分相对校正因子质量评价

Keywords

Xiao’er Qingre Huatan suppositoryhigh performance liquid chromatography-quantitative analysis of multi-components by single-markermulti-index componentsrelative correction factorquality evaluation

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