Objective: To verify the targeted regulation role of microRNA410 (miR-410) on expression of interleukin-13 (IL-13) mRNA. Methods: The binding sites of miR-410 in 3’ untranslated region (3’ UTR) of IL-13 mRNA were predicted by the bioinformatics software. IL-13 gene fragments binding miR-410 and mutant sequences were designed and synthesized. The wild and mutant luciferase reporter gene carriers were respectively constructed. miR-410 mimics or negative control of mimics were respectively co-transfected into 293T cells with wild or mutant luciferase reporter gene. The luciferase activity was detected to observe the effects of miR-410 on expression of IL-13 mRNA. Results: Bioinformatics analysis showed that there was binding site located in 3’ UTR of IL-13 mRNA. The electrophoresis showed that the wild and mutant luciferase reporter gene carriers were constructed successfully. Through the dual-luciferase reportier system

it was found that miR-410 could bind the wild-type IL-13 mRNA luciferase gene sequence and inhibit its expression

but had no effect on the mutant IL-13 mRNA luciferase expression (P<0.05). Conclusion: IL-13 mRNA can be targeted by miR-410.