Objective: To analyze the volatile components of Kechuan Jinshou Yuzu powder I

so as to provide reference for further study of traditional Chinese medicine compound preparation. Methods: Volatile components of Kechuan Jinshou Yuzu powder I were extracted by using headspace static heating extraction technology. Headspace sampling-gas chromatography-mass spectrometry (HS-GC-MS) technology was used for analysis. Agilent DB-5MS capillary column (0.25 m×0.25 mm×30 m) was used for separation. The programmed temperature was set as the initial temperature of 40 ℃

which was maintained for 3 min

then increased to 120 ℃ at 4 ℃/min

maintained for 5 min

and increased to 160 ℃ at 8 ℃/min

maintained for 5 min. The carrier gas was high-purity helium (purity ≥99.999%)

with the carrier gas flow rate of 1.0 mL/min. The splitting ratio was 100∶1

inlet temperature was 200℃ and injection volume was 1µL. Mass spectrometry conditions: ionization mode EI

bombardment voltage 70eV

positive ion mode

ion source temperature 230 ℃. Results: The HS-GC-MS map obtained from 10 samples identified 17 common peaks

including 2

3

5

6-tetraminepyrazine (No.12

3.34%)

D-limonene (No.8

16.26%)

α-pinene (No.3

6.94%) and other related substances. Conclusion: This study reveals the composition of volatile components of Kechuan Jinshou Yuzu powder I

among which the main effective components may be 2

3

5

6-tetramethylpyrazine

D-limonene and α-pinene

as well as transdermal absorption enhancers including myrcene

β-pinene and carene.