Objective: To establish the high performance liquid chromatography-fluorescence detection (HPLC-FLD) method for determination of bisphenol A (BPA) and 4-nonylphenol (4-NP) in plasma of of girls with precocious puberty. Methods: Shim-pack VP-ODS C18 chromatographic column (150 mm×4.6 mm

5 μm) was adopted

the mobile phase was acetonitrile∶water=70∶30

with flow rate of 1 mL/min

column temperature of 30 ℃

detection excitation wavelength of 275 nm

and emission wavelength of 315 nm. Content of BPA and 4-NP in plasma was detected by external standard method. Results: The linear relationship between BPA and 4-NP was good in the range from 1.45 to 5

937.50 ng/mL (r2=0.999

9

n=7) and from 1.53 to 6

250.00 ng/mL (r2=0.998

8

n=7). The RSD of intra-day and inter-day precision was <7.83%

and the recovery rate was >94.6%. Conclusion: This method is simple

rapid

sensitive and accurate

and is suitable for simultaneous determination of BPA and 4-NP in plasma and routine detection of clinical blood samples in girls with precocious puberty.