Objective: To investigate the bacterial culture spectrum of invasive catheter ends and drug resistance in pediatric intensive care unit (PICU)

and to provide reference for clinical prevention and treatment of nosocomial infection. Methods: From 2013 to 2015

retrospective investigation was conducted on the isolated bacterial strains and drug resistance of invasive catheter ends such as endotracheal intubation

deep vein catheterization and urinary catheterization in PICU of the Children’s Hospital of Nanjing Medical University. Results: A total of 218 positive cultures were obtained in three years

and 256 strains were isolated. The isolates were mainly 179 strains of G- bacteria (69.92%)

followed by 60 strains of G+ bacteria (23.44%) and 17 strains of fungi (6.64%). The top six isolates were respectively Acinetobacter baumannii (32.42%)

Klebsiella pneumoniae (17.58%)

Enterococcus faecalis (4.69%)

Candida albicans (4.30%)

Pseudomonas aeruginosa (4.30%) and methicillin-resistant Staphylococcus aureus (MRSA

3.91%). In vitro susceptibility testing showed that the main isolates were multi-drug resistant. In vitro drug susceptibility tests showed that the main isolates were multi-drug resistant and highly resistant to imipenem and third-generation cephalosporins. Piperacillin/tazobactam had strong antibacterial activity against P. aeruginosa

and the sensitivity rate was up to 100.00%. Only vancomycin and linezolidine had strong antibacterial activity against G+

and the sensitivity rate was up to 100.00%. Conclusion: In PICU

G-bacteria are the main isolates of bacterial culture of invasive catheter ends

and the bacteria are multi-drug resistant. E. faecalis and MRSA have a high proportion in G+ bacteria and should be highly value