REN Mei-ping1, LI Wan-ping2, LIU Ming-hua2, et al. Effects of verapamil on apoptosis and the Bcl-2/Bax expression of hepatic stellate cells. [J]. Journal of Chongqing Medical University 36(9):1037-1039(2011)
REN Mei-ping1, LI Wan-ping2, LIU Ming-hua2, et al. Effects of verapamil on apoptosis and the Bcl-2/Bax expression of hepatic stellate cells. [J]. Journal of Chongqing Medical University 36(9):1037-1039(2011) DOI: 10.13406/j.cnki.cyxb.2011.09.038.
Effects of verapamil on apoptosis and the Bcl-2/Bax expression of hepatic stellate cells
Objective:To study the effects of Verapamil on apoptosis and the expression of Bcl-2/Bax of human hepatic stellate cells(HSCs).Methods:HSCs were treated with different concentrations of Verapamil for 48 h.The inhibitory rate of Verapamil on HSCs was determined by CCK-8 assay;the rate of apoptosis was detected by flow cytometry with Annexin-V/PI staining;and the expression of Bcl-2/Bax was tested by SABC of immunohistochemical method.Results:Different concentrations of Verapamil had certain inhibitory efffct on HSCs cell proliferation in a dose-dependent manner
and the IC50 value was(0.15±0.01) mol/L.The apoptosis of HSCs was induced by different concentrations of Verapamil
which was statistically different from the negative control group(P<0.05).The expression of Bcl-2 of HSCs cells was decreased and the expression of Bax was increased after treatment of Verapamil
which was statistically different from the negative control group(P<0.05).Conclusion:Verapamil can inhibit the proliferation and induce the apoptosis of HSCs cells
and its mechanism might be related to decreasing the expression of Bcl-2 and increasing the expression of Bax.