Screening differentially expressed proteins of cleft palates induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin in mice

LIU Li-ling; FU Yue-xian; PU Ya-lan; GAN Li-qiang

doi:10.13406/j.cnki.cyxb.2011.09.037

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Screening differentially expressed proteins of cleft palates induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin in mice

更新时间：2024-01-17

- Screening differentially expressed proteins of cleft palates induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin in mice
- Journal of Chongqing Medical University Vol. 36, Issue 9, Pages: 1087-1090(2011)
- 作者机构：
  
  重庆医科大学儿童发育疾病研究省部共建教育部重点实验室
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2011.09.037
  CLC： R782.2
- Published：2011，
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[1]刘丽玲,傅跃先,蒲亚兰,甘立强.2,3,7,8-四氯二苯二噁英诱导腭裂发生的差异蛋白筛选[J].重庆医科大学学报,2011,36(09):1087-1090.

LIU Li-ling, FU Yue-xian, PU Ya-lan, et al. Screening differentially expressed proteins of cleft palates induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin in mice. [J]. Journal of Chongqing Medical University 36(9):1087-1090(2011)
[1]刘丽玲,傅跃先,蒲亚兰,甘立强.2,3,7,8-四氯二苯二噁英诱导腭裂发生的差异蛋白筛选[J].重庆医科大学学报,2011,36(09):1087-1090. DOI： 10.13406/j.cnki.cyxb.2011.09.037.

LIU Li-ling, FU Yue-xian, PU Ya-lan, et al. Screening differentially expressed proteins of cleft palates induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin in mice. [J]. Journal of Chongqing Medical University 36(9):1087-1090(2011) DOI： 10.13406/j.cnki.cyxb.2011.09.037.

摘要

目的:以蛋白质组学方法筛选2

8-四氯二苯二噁英（2

8-tetrachlorodibenzo-p-dioxin

TCDD）致先天性腭裂发生的差异表达蛋白。方法:以TCDD诱导建立胎鼠先天性腭裂模型

行腭部的大体解剖及组织学检查。取实验组和对照组胎鼠的腭组织

分别提取组织总蛋白。通过双向电泳和质谱分析

筛选出实验组和对照组腭组织的差异表达蛋白

并用免疫组化对鉴定出的相关蛋白质进行验证。结果:成功诱导胎鼠腭裂模型。筛选出10种差异表达的蛋白

其中7个蛋白点在试验组上调

3个蛋白点在实验组下调。过氧化物还原酶1（Peroxiredoxin 1

Prx 1）与鸟嘌呤核苷酸解离抑制剂（GDP-dissociation inhibitor

GDI）在实验组表达明显增强。结论:Prx 1、GDI表达的明显增强可能与TCDD所致胎鼠先天性腭裂有关。

Abstract

Objective:To identify the differentially expressed proteins of 2

8-tetrachloro-dibenzo-p-dioxin(2

8-TCDD)induced cleft palate by using two-dimensional gel electrophoresis(2-DE) and mass spectrometry.Methods:On E12

8-TCDD(64 μg/kg) and corn oil(control group) were administered to time-pregnant C57BL/6J mice.Anatomical and histological changes of palates in mouse fetuses were studied on E18.Total proteins were extracted from palate tissue of fetuses from both groups.The total proteins were separated by two-dimensional gel electrophoresis.The differentially expressed proteins between the two groups were compared using image analysis software.The peptide mass fingerprintings(PMF) was acquired after matrix assisted laser desorption/ionization time of flight mass spectrometry(MALDI-TOF-MS)and the proteins were identified by data searching in the Mascot database.The expression of the differential protein was confirmed by immunohistochemistry.Results:Incidence of cleft palate in the experimental group was 100%.Comparative analysis of 2-DE maps revealed 34 differentially expressed proteins between the two groups.Ten differential proteins were further identified by mass spectrometry

of which 7 showed significantly higher volumes in experimental group than in control group and 3 showed significantly higher volumes in control group than in experimental group.Peroxiredoxin 1 protein and guanine nucleotide dissociation inhibitors were up-regulated in experimental group.Conclusion:Up-regulated peroxiredoxin 1 protein and guanine nucleotide dissociation inhibitors may be associated with cleft palate in mice induced by 2

8-TCDD.

关键词

2378-四氯二苯二噁英腭裂蛋白质组学

Keywords

2378-tetrachloro-dibenzo-p-dioxincleft palateproteomics

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