Effect of SB203580 on apoptosis and expression of bcl-2 protein in rat hepatic stellate cells

ZHUO Qiang1; ZHENG Ren-yuan1; YE Ping1; TANG Wen1; MEI Zhe-chuan2; JIANG Ming-de1

doi:10.13406/j.cnki.cyxb.2011.09.014

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Effect of SB203580 on apoptosis and expression of bcl-2 protein in rat hepatic stellate cells

更新时间：2024-01-17

- Effect of SB203580 on apoptosis and expression of bcl-2 protein in rat hepatic stellate cells
- Journal of Chongqing Medical University Vol. 36, Issue 9, Pages: 1054-1056(2011)
- 作者机构：
  
  1. 中国人民解放军成都军区总医院消化内科
  2. 重庆医科大学附属第二医院消化内科
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2011.09.014
  CLC： R575.2
- Published：2011，
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[1]卓强,郑仁源,叶平,唐文,梅浙川,蒋明德.SB203580对大鼠肝星状细胞凋亡及bcl-2蛋白表达影响[J].重庆医科大学学报,2011,36(09):1054-1056.

ZHUO Qiang1, ZHENG Ren-yuan1, YE Ping1, et al. Effect of SB203580 on apoptosis and expression of bcl-2 protein in rat hepatic stellate cells. [J]. Journal of Chongqing Medical University 36(9):1054-1056(2011)
[1]卓强,郑仁源,叶平,唐文,梅浙川,蒋明德.SB203580对大鼠肝星状细胞凋亡及bcl-2蛋白表达影响[J].重庆医科大学学报,2011,36(09):1054-1056. DOI： 10.13406/j.cnki.cyxb.2011.09.014.

ZHUO Qiang1, ZHENG Ren-yuan1, YE Ping1, et al. Effect of SB203580 on apoptosis and expression of bcl-2 protein in rat hepatic stellate cells. [J]. Journal of Chongqing Medical University 36(9):1054-1056(2011) DOI： 10.13406/j.cnki.cyxb.2011.09.014.

摘要

目的:通过观察P38MAPK信号传导通路特异抑制剂SB203580对乙醛刺激的大鼠肝星状细胞（Hepatic stellate cells

HSCs）凋亡和bcl-2蛋白表达的影响

探讨P38MAPK信号传导通路与bcl-2蛋白在HSCs中的作用

为肝纤维化的治疗提供理论依据。方法:不同浓度SB203580处理乙醛刺激的HSC-T6

利用MTT法检测SB203580对细胞的抑制作用

ELISA酶联法检测细胞凋亡情况

Western blot法观察bcl-2蛋白表达水平。结果:SB203580抑制乙醛刺激的HSC-T6存活和诱导其凋亡

呈剂量依赖性（F=13.388和F=155.351

P<0.01）;随着SB203580浓度增加

bcl-2蛋白表达逐渐降低（F=691.470

P<0.01）。结论:不同浓度SB203580能诱导乙醛刺激的HSC-T6凋亡

其作用机制可能与下调bcl-2蛋白表达有关。

Abstract

Objective:To investigate the relationship of the apoptosis of rat hepatic stellate cells induced by SB203580 and expression of bcl-2 protein.Methods:HSC-T6 stimulated by acetaldehyde was exposed to SB203580 at different concentrations.The inhibitory effect of SB203580 on cells was detected by MTT.The apoptosis of HSC-T6 was detected by ELISA;the positive expression rate of bcl-2 protein was observed by Western blot.Results:SB203580 induced the apoptosis of HSC-T6 stimulated by acetaldehyde

and all showed the does-dependent trend(F=16.388 and F=155.351

P<0.01).With the concentration of SB203580 increasing

the positive expression rate of bcl-2 protein decreased gradually.(F=382.834

P<0.01).Conclusion:The apoptosis of HSC-T6 stimulated by acetaldehyde is obviously enhanced by SB203580

which maybe result from the down-regulated expression of bcl-2.

关键词

SB203580乙醛肝星状细胞Bcl-2凋亡

Keywords

SB203580acetaldehydehepatic stellate cellsBcl-2apoptosis

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