Effects of endothelial microparticles on expressions of VCAM-1 and ICAM-1 in human umbilical vein endothelial

LU Yong-guang1; SU Qiang2; ZENG Xiao-cong2; YAN Hua1; HUANG Jun-zhang1; FU Chun-hui1

doi:10.13406/j.cnki.cyxb.2011.09.013

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Effects of endothelial microparticles on expressions of VCAM-1 and ICAM-1 in human umbilical vein endothelial

更新时间：2024-01-17

- Effects of endothelial microparticles on expressions of VCAM-1 and ICAM-1 in human umbilical vein endothelial
- Journal of Chongqing Medical University Vol. 36, Issue 9, Pages: 1063-1065(2011)
- 作者机构：
  
  1. 钦州市第二人民医院心内科
  2. 广西医科大学第一附属医院心内科
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2011.09.013
  CLC： R541.4
- Published：2011，
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[1]陆永光,苏强,曾晓聪,严华,黄军章,符春晖.内皮细胞微粒对人脐静脉内皮细胞VCAM-1和ICAM-1表达的影响[J].重庆医科大学学报,2011,36(09):1063-1065.

LU Yong-guang1, SU Qiang2, ZENG Xiao-cong2, et al. Effects of endothelial microparticles on expressions of VCAM-1 and ICAM-1 in human umbilical vein endothelial. [J]. Journal of Chongqing Medical University 36(9):1063-1065(2011)
[1]陆永光,苏强,曾晓聪,严华,黄军章,符春晖.内皮细胞微粒对人脐静脉内皮细胞VCAM-1和ICAM-1表达的影响[J].重庆医科大学学报,2011,36(09):1063-1065. DOI： 10.13406/j.cnki.cyxb.2011.09.013.

LU Yong-guang1, SU Qiang2, ZENG Xiao-cong2, et al. Effects of endothelial microparticles on expressions of VCAM-1 and ICAM-1 in human umbilical vein endothelial. [J]. Journal of Chongqing Medical University 36(9):1063-1065(2011) DOI： 10.13406/j.cnki.cyxb.2011.09.013.

摘要

目的:探讨内皮细胞微粒（Endothelial microparticles

EMPs）对人脐静脉内皮细胞（Human umbilical vein endothelialcells

HUVECs）血管细胞粘附分子-1（Vascular cellular adhesion molecule-1

VCAM-1）和细胞间粘附分子-1（Intercellular adhe-sion molecule 1

ICAM-1）表达的影响。方法:取生长良好的第4、5代HUVECs

将细胞以1×105个/ml密度接种于5 cm2的培养皿中

待细胞生长近80%融合时进行干预。按0、1×102、1×103、1×104、1×105个/ml浓度的EMPs进行分组及刺激

每组12个样本。在共同培养24 h后收集细胞。分别采用实时荧光定量聚合酶链反应和蛋白免疫印迹方法检测VCAM-1和ICAM-1 mRNA和蛋白的表达。结果:HUVECs受EMPs刺激

VCAM-1和ICAM-1 mRNA及蛋白表达显著增加

且EMPs的这种作用呈浓度依赖性增强（均P<0.05）。结论:EMPs能上调HUVECs VCAM-1和ICAM-1的表达

EMPs不仅是内皮功能障碍的标记物

还能加重内皮功能损害。

Abstract

Objective:To examine the effects of endothelial microparticles(EMPs)on the expressions of vascular cell adhesion molecule 1(VCAM-1) and intercellular adhesion molecule(ICAM-1) in human umbilical vein endothelial cells(HUVECs).Methods:HUVECs at passage 4 to 5 were incubated for 24 h with EMPs(0

1×102

1×103

1×104

1×105/ml).Quantitative real-time polymerase chain reaction and Western blotting were used to detect expressions of mRNA and protein of ICAM-1 and VCAM-1 respectively.Results:Both two detection methods showed that EMPs stimulation significantly induced the mRNA and protein expressions of VCAM-1 and ICAM-1 in HUVECs in a dose-dependent manner.Conclusion:EMPs can induce the expressions of VCAM-1 and ICAM-1 in a dose-dependent manner.These findings suggest that EMPs may have adverse effects in modulating inflammatory response in atherosclerosis.

关键词

内皮细胞微粒脐静脉内皮细胞血管细胞粘附分子-1细胞间粘附分子-1

Keywords

endothelial microparticleshuman umbilical vein endothelial cellsvascular cell adhesion molecule 1intercellular adhesion molecule 1

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