HPLC determination of the content and entrapment efficiency of artemisia ketone in compound tretinoin liposome

YANG Shi-yan1; HE Bing2; FENG Wen-yu2

doi:10.13406/j.cnki.cyxb.2011.08.042

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HPLC determination of the content and entrapment efficiency of artemisia ketone in compound tretinoin liposome

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- HPLC determination of the content and entrapment efficiency of artemisia ketone in compound tretinoin liposome
- Journal of Chongqing Medical University Vol. 36, Issue 8, Pages: 956-958(2011)
- 作者机构：
  
  1. 泸州医学院附属中医医院超声影像科
  2. 泸州医学院药物研究中心
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2011.08.042
  CLC： R927.2
- Published：2011，
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[1]杨世艳,何兵,冯文宇.HPLC测定复方维A酸脂质体中蒿酮的含量和包封率[J].重庆医科大学学报,2011,36(08):956-958.

YANG Shi-yan1, HE Bing2, FENG Wen-yu2. HPLC determination of the content and entrapment efficiency of artemisia ketone in compound tretinoin liposome. [J]. Journal of Chongqing Medical University 36(8):956-958(2011)
[1]杨世艳,何兵,冯文宇.HPLC测定复方维A酸脂质体中蒿酮的含量和包封率[J].重庆医科大学学报,2011,36(08):956-958. DOI： 10.13406/j.cnki.cyxb.2011.08.042.

YANG Shi-yan1, HE Bing2, FENG Wen-yu2. HPLC determination of the content and entrapment efficiency of artemisia ketone in compound tretinoin liposome. [J]. Journal of Chongqing Medical University 36(8):956-958(2011) DOI： 10.13406/j.cnki.cyxb.2011.08.042.

摘要

目的:建立复方维A酸脂质体中蒿酮的含量和包封率测定的高效液相色谱（High performance liquid chromatography

HPLC）法。方法:色谱柱:迪马Kromasil C18柱（250 mm×4.6 mm

5μm）;流动相:乙腈-水（75∶25）;检测波长:242 nm;柱温:30℃;流速:0.8 ml/min;进样量:10μl。结果:蒿酮在25.16～251.60μg/ml范围内具有良好的线性关系（r=0.999 9）

平均回收率为99.53%

RSD为0.72%。结论:该方法简便快速、准确

具有良好的重复性和回收率

可作为该制剂中蒿酮的定量分析方法。

Abstract

Objective:To establish a HPLC method for the content and entrapment efficiency determination of artemisia ketone in compound tretinoin liposome.Methods:The separation was carried out on a Dikma Kromasil C18 column(250 mm×4.6mm

5 μm).The mobile phase was acetonitrile-water(75∶25).The detective wavelength was set at 242 nm.The column temperature was 30℃.The flow rate was 0.8 ml/min and the sample size was 10 μl.Results:The calibration curve showed good linearity over the range of 25.16～251.60 μg/ml(r=0.999 9).The average recovery was 99.53%with RSD of 0.72%.Conclusion:This method is simple

rapid

accurate and with good repeatability and recovery.It can be used as a quantitative analysis method for this preparation.

关键词

高效液相色谱复方维A酸脂质体蒿酮含量测定包封率

Keywords

HPLCcompound tretinoin liposomeartemisia ketonecontent determinationentrapment efficiency

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