The experimental study on secondary lung injury by aspiration in severe brain injury rats

QUAN Ming-tao1; JIANG Zhi-xia1; REN Guang-yang1; SHI Jiu-jun1; LUO Yi1; CHENG Hua-gang2

doi:10.13406/j.cnki.cyxb.2011.08.023

您当前的位置：

首页 >

文章列表页 >

The experimental study on secondary lung injury by aspiration in severe brain injury rats

更新时间：2024-01-17

- The experimental study on secondary lung injury by aspiration in severe brain injury rats
- Journal of Chongqing Medical University Vol. 36, Issue 8, Pages: 921-923(2011)
- 作者机构：
  
  1. 遵义医学院附属医院ICU
  2. 遵义医学院附属医院神经科
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2011.08.023
  CLC： R651.1
- Published：2011，
扫描看全文
[1]权明桃,江智霞,任光阳,施久军,罗祎,程华刚.重度脑损伤误吸大鼠继发肺损伤的实验研究[J].重庆医科大学学报,2011,36(08):921-923.

QUAN Ming-tao1, JIANG Zhi-xia1, REN Guang-yang1, et al. The experimental study on secondary lung injury by aspiration in severe brain injury rats. [J]. Journal of Chongqing Medical University 36(8):921-923(2011)
[1]权明桃,江智霞,任光阳,施久军,罗祎,程华刚.重度脑损伤误吸大鼠继发肺损伤的实验研究[J].重庆医科大学学报,2011,36(08):921-923. DOI： 10.13406/j.cnki.cyxb.2011.08.023.

QUAN Ming-tao1, JIANG Zhi-xia1, REN Guang-yang1, et al. The experimental study on secondary lung injury by aspiration in severe brain injury rats. [J]. Journal of Chongqing Medical University 36(8):921-923(2011) DOI： 10.13406/j.cnki.cyxb.2011.08.023.

摘要

目的:探讨重度脑损伤误吸胃液致肺损伤的机制。方法:SD雄性大鼠50只

随机分为对照组（10只）和实验组（40只）。实验组采用改进型Marmarou模型方法致大鼠重度脑损伤

再经气管内滴入胃液

制作重度脑损伤误吸模型。分别在造模后2、6、24和48 h各处死10只大鼠收集标本

采用酶联免疫吸附试验（Enzyme linked immunosorbent assay

ELISA）法检测大鼠血浆中性粒细胞弹性蛋白酶（Neutrophil elastase

NE）含量、支气管肺泡灌洗液（Bronchoalveolar lavage fluid

BALF）中NE和白介素-8（Interleukin-8

IL-8）含量;免疫透射比浊法测定BALF中白蛋白浓度;光学显微镜观察肺组织病理改变情况。结果:实验组BALF和血浆中NE含量2 h开始升高

与对照组比较差异有统计学意义（P<0.05）。BALF中NE升高幅度较血浆明显;BALF中IL-8含量在2h开始升高

同时BALF中白蛋白浓度也明显升高（P<0.05）;BALF中NE、IL-8与白蛋白浓度呈正相关（P<0.01）。结论:重度脑损伤误吸可造成肺组织的炎症反应

NE、IL-8参与肺内炎性反应

造成肺组织损伤。

Abstract

Objective:To investigate gastric aspiration induced lung injury in severe brain injury.Methods:50 male SD rats were randomly divided into control group(n=10) and experimental group(n=40).The severe brain injury in experimental group was made by an improved method of Marmarou model

and then the aspiration models were made by intratracheal instillation of gastric juice.Each ten rats were killed to collect samples at 2 h

6 h

24 h and 48 h separately after the models were made.Plasma neutrophil elastase(Neutrophil Elastase

NE) content

the NE content and interleukin-8(IL-8) content in bronchoalveolar lavage fluid(BALF) were detected by using enzyme-linked immunosorbent assay(ELISA);immune turbidimetric determination was used for the albumin concentration in BALF;and optical microscope for pathological changes in lung tissue.Results:BALF and plasma NE levels began to increase at 2 h

which was significant compared with the control group(P<0.05).NE in BALF increased at a significantly higher rate than in plasma;IL-8 level in BALF began to increase at 2h

and meanwhile

BALF albumin concentration was significantly increased(P<0.05);NE

IL-8 in BALF and albumin concentration had positive correlation(P<0.01).Conclusion:Aspiration after severe brain injury can cause inflammation in lung tissue

and NE and IL-8 take part in pulmonary inflammatory response

resulting in lung injury.

关键词

重度脑损伤急性肺损伤中性粒细胞弹性蛋白酶

Keywords

severe brain injuryacute lung injuryneutrophil elastase

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

TNF-α downregulates the expression of tight junction proteins ZO-1 and Claudin-5 in rat pulmonary microvascular endothelial cells

Research progress of autophagy in lung inflammatory diseases

Study of pulmonary surfactant and surfactant protein in rats with LPS-induced acute lung injury

The alert role of Clara cell secretory protein in serum from patients in the developing course of acute respiratory distress syndrome

Acute lung injury induced by endotoxic shock and imbalance of Th1/Th2 type of cytokines

Related Author

Ma Xigang

Ma Shenmao

Shi Guocui

Bai Jijia

Liu Qiong

Duan Yushan

王兴勇

卢仲毅

Related Institution

Department of Respiratory Medicine,Cangzhou People's Hospital

Department of Critical Care Medicine,General Hospital of Ningxia Medical University

Department of Emergency and Critical Care Medicine,The First Affiliated Hospital of Chongqing Medical University

重庆医科大学儿科学院PICU

Department of PICU,College of Pediatrics,Chongqing Medical University

⁰