Inhibition effect of tetrandrine on division of fibroblast from keloid

CAO Zhi-dong1; SHI Chong-rong2; HUANG Chong-ben2; GOU Jing-yue1; LI Bang-chun1; TIAN Kai-xi1

doi:10.13406/j.cnki.cyxb.2011.08.011

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Inhibition effect of tetrandrine on division of fibroblast from keloid

更新时间：2024-01-17

- Inhibition effect of tetrandrine on division of fibroblast from keloid
- Journal of Chongqing Medical University Vol. 36, Issue 8, Pages: 965-967(2011)
- 作者机构：
  
  1. 重庆市第四人民医院
  2. 重庆市急救医学研究所骨科
  3. 重庆医科大学附属第一医院烧伤整形外科
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2011.08.011
  CLC： R285
- Published：2011，
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[1]曹治东,石崇荣,黄崇本,苟景跃,李邦春,田开熙.汉防己甲素对瘢痕成纤维细胞有丝分裂的调控[J].重庆医科大学学报,2011,36(08):965-967.

CAO Zhi-dong1, SHI Chong-rong2, HUANG Chong-ben2, et al. Inhibition effect of tetrandrine on division of fibroblast from keloid. [J]. Journal of Chongqing Medical University 36(8):965-967(2011)
[1]曹治东,石崇荣,黄崇本,苟景跃,李邦春,田开熙.汉防己甲素对瘢痕成纤维细胞有丝分裂的调控[J].重庆医科大学学报,2011,36(08):965-967. DOI： 10.13406/j.cnki.cyxb.2011.08.011.

CAO Zhi-dong1, SHI Chong-rong2, HUANG Chong-ben2, et al. Inhibition effect of tetrandrine on division of fibroblast from keloid. [J]. Journal of Chongqing Medical University 36(8):965-967(2011) DOI： 10.13406/j.cnki.cyxb.2011.08.011.

摘要

目的:探讨汉防己甲素对瘢痕成纤维细胞有丝分裂的影响。方法:将不同浓度梯度的汉防己甲素与瘢痕成纤维细胞体外共培养

不同时间点分别取细胞标本检测其分裂指数

并使用流式细胞仪检测其细胞周期及不同周期细胞分布。将以上检测值与空白对照及阳性对照比较。结果:汉防己甲素组在各个时间点的分裂指数均较空白对照组明显下降

使峰值时间缩短

具有作用浓度及时间依赖性

汉防己甲素组G0-G1期细胞比例降低

S期及G2-M期细胞比例明显增多

S期与G2-M期时相时间明显延长。结论:汉防己甲素使成纤维细胞分裂停滞于G2-M期

抑制细胞分裂

且具有明显浓度及时间依赖性。

Abstract

Objective:To probe the effect of tetrandrine on mitosis of scar fibroblast.Methods:Fibroblast was cultured with various concentrations of tetrandrine

then the cultured fibroblast samples were gathered on different days of time gradient.Mitosis index was determined by light microscopy.Cell cycle and its distributions were analyzed by flow cytometer.Results:Mitosis index of tetrandrine group was reduced obviously compared with blank group at all time points and the peak time was shortened in a dose-and time-dependent manner.In the flow cytometry analysis

it was found that tetrandrine could promote fibroblast to enter S and G2-M phase from G0-G1 phase and extend the time of S & G2-M phase distinctly.Conclusion:Tetrandrine can block the division of fibroblast at G2-M phase in a dose-and time-dependent manner.

关键词

汉防己甲素成纤维细胞增殖性瘢痕细胞分裂细胞周期

Keywords

tetrandrinefibroblastkeloidcell divisioncell cycle

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