The expressions of multidrug resistance gene1(mdr1) and NF-κB of leukemia K562/A02 cell and the intervention function research of Jeduhuayu Chinese herbal serum

MA Wu-kai; YAO Xue-ming; TANG Fang; WANG Ying; HUANG Ying; AN Yang; HUANG Li-ming

doi:10.13406/j.cnki.cyxb.2011.08.009

您当前的位置：

首页 >

文章列表页 >

The expressions of multidrug resistance gene1(mdr1) and NF-κB of leukemia K562/A02 cell and the intervention function research of Jeduhuayu Chinese herbal serum

更新时间：2024-01-17

- The expressions of multidrug resistance gene1(mdr1) and NF-κB of leukemia K562/A02 cell and the intervention function research of Jeduhuayu Chinese herbal serum
- Journal of Chongqing Medical University Vol. 36, Issue 8, Pages: 897-900(2011)
- 作者机构：
  
  贵阳中医学院第二附属医院血液风湿科
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2011.08.009
  CLC： R285
- Published：2011，
扫描看全文
[1]马武开,姚血明,唐芳,王莹,黄颖,安阳,黄礼明.解毒化瘀药对白血病K562/A02耐药细胞mdr1耐药基因、核因子κB信号影响的研究[J].重庆医科大学学报,2011,36(08):897-900.

MA Wu-kai, YAO Xue-ming, TANG Fang, et al. The expressions of multidrug resistance gene1(mdr1) and NF-κB of leukemia K562/A02 cell and the intervention function research of Jeduhuayu Chinese herbal serum. [J]. Journal of Chongqing Medical University 36(8):897-900(2011)
[1]马武开,姚血明,唐芳,王莹,黄颖,安阳,黄礼明.解毒化瘀药对白血病K562/A02耐药细胞mdr1耐药基因、核因子κB信号影响的研究[J].重庆医科大学学报,2011,36(08):897-900. DOI： 10.13406/j.cnki.cyxb.2011.08.009.

MA Wu-kai, YAO Xue-ming, TANG Fang, et al. The expressions of multidrug resistance gene1(mdr1) and NF-κB of leukemia K562/A02 cell and the intervention function research of Jeduhuayu Chinese herbal serum. [J]. Journal of Chongqing Medical University 36(8):897-900(2011) DOI： 10.13406/j.cnki.cyxb.2011.08.009.

摘要

目的:探讨解毒化瘀药含药血清对白血病K562/A02细胞mdr1耐药基因、核因子κB（Nuclear factor kappa B

NF-κB）信号基因表达的影响。方法:采用半定量逆转录聚合酶链反应法检测解毒化瘀药含药血清处理后K562/A02细胞mdr1基因的表达;Western blot法检测NF-κB/P65、NF-κB/P50、IκBα的表达。结果:解毒化瘀药含药血清能够降低K562/A02耐药细胞NF-κB/P65、NF-κB/P50、IκBα表达

降低mdr1耐药基因的表达。结论:解毒化瘀药对K562/A02细胞的耐药逆转作用可能是通过阻断NF-κB信号通路

影响mdr1耐药基因的表达起作用的。

Abstract

Objective:To discuss the influence of Jieduhuayu Chinese herbal serum on the expression of multidrug resistance gene1(Mdr1) of leukemia K562/A02 cell and the expression of NF-κB(nuclear factor kappa B).Methods:Semi-quantitative reverse transcriptase polymerase chain reaction method was used to examine the expression of Mdr1 gene after K562/A02 cell receiving the treatment of Jieduhuayu Chinese herbal serum;Western blotting technology was used to examine of the expressions of NF-κB/P65

NF-κB/P50

and IκBα.Results:The expressions of NF-κB/P65

NF-κB/P50

and IκBα and the expression of multidrug resistance gene1 of leukemia K562/A02 cell could be decreased by Jieduhuayu Chinese herbal serum.Conclusion:Jieduhuayu Chinese herbal serum can reverse multidrug resistance in leukemia cell by blocking NF-κB signal pathway

and influence the expression of multidrug resistance gene1(mdr1).

关键词

白血病多药耐药K562/A02细胞核因子κB信号解毒化瘀药

Keywords

leukemiamultidrug resistanceK562/A02 cellnuclear factor kappa B pathwayJieduhuayu Chinese herbal medicine

references

Views

156

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Factors affecting the quality of life in children with leukemia and the relevant research progress at home and abroad

Effect of artesunate on autophagy of leukemic K562 cells and its mechanism

RCIA AND RBC SOD ACTIVITY IN PATIENTS WITH LEUKEMIA AND MALIGNANT LYMPHOMA

DETERMINATION OF ACUTE PROTEIN AND SIALIC ACID IN PATIENTS WITH ACUTE LEUKEMIA

MORPHOLOGICAL AND IMMUNOPHENOTYPICSTUDIES ON ACUTE LEUKEMIA

Related Author

Shi Lin

Mo Lin

He Yun

Liu Yang

Wang Zijuan

Zhang Ping

Yang Guicun

Chen Hongxia

Related Institution

the Third Surgical Ward,the Children’s Hospital,Chongqing Medical University

Special Needs Outpatient,the Children’s Hospital,Chongqing Medical University

Department of Hematology,the Children’s Hospital,Chongqing Medical University,Ministry of Education Key Laboratory of Child Development and Disorders,China International Science andTechnology Cooperation Base of Child Development and Critical Disorders,Chongqing Key Laboratory of Pediatrics

重庆医科大学第一医院内科血液组重庆医科大学检验科

重庆医科大学检验科

⁰