Determination of total saponins in astragalus membranaceus by ultraviolet spectroscopy

LI Chun-hong1; TIAN Ji1; HE Bing1; XIAO Shun-han2

doi:10.13406/j.cnki.cyxb.2011.08.008

您当前的位置：

首页 >

文章列表页 >

Determination of total saponins in astragalus membranaceus by ultraviolet spectroscopy

更新时间：2024-01-17

- Determination of total saponins in astragalus membranaceus by ultraviolet spectroscopy
- Journal of Chongqing Medical University Vol. 36, Issue 8, Pages: 954-956(2011)
- 作者机构：
  
  1. 泸州医学院药物及功能性食品研究中心
  2. 泸州医学院药学院
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2011.08.008
  CLC： R284
- Published：2011，
扫描看全文
[1]李春红,田吉,何兵,肖顺汉.紫外分光光度法测定黄芪总黄酮的含量[J].重庆医科大学学报,2011,36(08):954-956.

LI Chun-hong1, TIAN Ji1, HE Bing1, et al. Determination of total saponins in astragalus membranaceus by ultraviolet spectroscopy. [J]. Journal of Chongqing Medical University 36(8):954-956(2011)
[1]李春红,田吉,何兵,肖顺汉.紫外分光光度法测定黄芪总黄酮的含量[J].重庆医科大学学报,2011,36(08):954-956. DOI： 10.13406/j.cnki.cyxb.2011.08.008.

LI Chun-hong1, TIAN Ji1, HE Bing1, et al. Determination of total saponins in astragalus membranaceus by ultraviolet spectroscopy. [J]. Journal of Chongqing Medical University 36(8):954-956(2011) DOI： 10.13406/j.cnki.cyxb.2011.08.008.

摘要

目的:建立黄芪总黄酮的含量测定方法。方法:采用紫外分光光度法测定黄芪总黄酮。结果:总黄酮在0.025～0.5 mg范围内具有良好的线性关系（R=0.999 9）

回收率为98.27%

相对标准偏差（Relative standard deviation

RSD）为0.27%。结论:该方法操作简便、快速、有效、灵敏、准确

具有良好的重复性和回收率

可作为黄芪总黄酮含量的测定方法。

Abstract

Objective:To establish the determination method of total saponins in astragalus membranaceus.Methods:The content of total saponins was detected by UV Spectroscopy.Results:The calibration curve showed good linearity over the range of 0.025～0.5 mg(R=0.999 9).The average recovery was 98.27% with RSD 0.27%.Conclusion:This method is simple

rapid

efficient

sensitive

accurate

reproducibility and specificity

and can be used as a quantitative analysis method for this preparation.

关键词

黄芪总黄酮紫外分光光度法

Keywords

astragalus membranaceustotal saponinsUV Spectroscopy

references

Views

1326

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

INFLUENCE OF ASTRAGALUS MEMBRANACEUS AND EUCOMMIA ULMOIDES ON IMPAIRED PH AGOCYTIC FUNCTION OF MACROPHAGES IN STREPTOZOTOCIN-INDUCED DIABETIC MICE

αEffect of high dose of astragalus membranaceus on serum TNF- ,IL-6 and cellular immune function in patients with chronic glomerulonephritis

The effect of Astragalus membranaceus on serum NO in patients with chronic glomerulonephritis at pre-renal failure phase

Related Author

梁亚明

舒昌达

何军

陈莉芬

李荣亨

曹文富

张晓恒

高渝军

Related Institution

重庆医科大学第一医院内科内分泌

重庆医科大学第一医院内科内分泌研究生

成都中医学院附属医院内科

重庆医科大学第二临床学院神经内科

重庆医科大学临床学院中西医结合科

⁰