Effects of angelica polysaccharides on the cell cycle and cell apoptosis in bone marrow mononuclear cells of radiation injured mice

ZHANG Yan1; RUI Yong-jun1; MI Jing-yi1; WU Hong2

doi:10.13406/j.cnki.cyxb.2011.08.005

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Effects of angelica polysaccharides on the cell cycle and cell apoptosis in bone marrow mononuclear cells of radiation injured mice

更新时间：2024-01-17

- Effects of angelica polysaccharides on the cell cycle and cell apoptosis in bone marrow mononuclear cells of radiation injured mice
- Journal of Chongqing Medical University Vol. 36, Issue 8, Pages: 913-916(2011)
- 作者机构：
  
  1. 无锡市第九人民医院
  2. 无锡手外科医院实验中心
  3. 重庆医科大学基础医学院组织与胚胎学教研室
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2011.08.005
  CLC： R285.5
- Published：2011，
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[1]张雁,芮永军,糜菁熠,吴宏.当归多糖对放射损伤小鼠骨髓单个核细胞细胞周期及细胞凋亡的影响[J].重庆医科大学学报,2011,36(08):913-916.

ZHANG Yan1, RUI Yong-jun1, MI Jing-yi1, et al. Effects of angelica polysaccharides on the cell cycle and cell apoptosis in bone marrow mononuclear cells of radiation injured mice. [J]. Journal of Chongqing Medical University 36(8):913-916(2011)
[1]张雁,芮永军,糜菁熠,吴宏.当归多糖对放射损伤小鼠骨髓单个核细胞细胞周期及细胞凋亡的影响[J].重庆医科大学学报,2011,36(08):913-916. DOI： 10.13406/j.cnki.cyxb.2011.08.005.

ZHANG Yan1, RUI Yong-jun1, MI Jing-yi1, et al. Effects of angelica polysaccharides on the cell cycle and cell apoptosis in bone marrow mononuclear cells of radiation injured mice. [J]. Journal of Chongqing Medical University 36(8):913-916(2011) DOI： 10.13406/j.cnki.cyxb.2011.08.005.

摘要

目的:探讨当归多糖（Angelica polysaccharides

APS）对放射损伤小鼠骨髓单个核细胞（Bone marrow mononuclear cells

BMNCs）细胞周期及细胞凋亡的影响

旨在阐明APS防护辐射性造血损伤的分子机制。方法:BALB/c小鼠随机分为3组:正常对照组、生理盐水（NS）组和APS组。NS组和APS组小鼠全身均匀照射X射线

照射后腹腔注射给药NS和8 mg/kg APS

0.2ml/（次.d）

连续6 d

第7天计数外周血WBC、RBC、PLT及BMNCs;RT-PCR方法检测小鼠BMNCs凋亡相关基因p53mRNA的表达水平;流式细胞术检测小鼠BMNCs的细胞周期和凋亡率。结果:照射后第7天APS组外周血WBC、RBC、PLT及BMNCs计数明显高于NS组;BMNCs的p53mRNA的表达水平、G0/G1期细胞比例和凋亡率较NS组明显下降。结论:APS能通过降低放射损伤小鼠BMNCs p53mRNA的表达水平

改善BMNCs细胞周期紊乱

抑制细胞凋亡

促进造血恢复。

Abstract

Objective:To explore the effects of angelica polysaccharides(APS) on the cell cycle and cell apoptosis rate in bone marrow mononuclear cells(BMNCs) of radiation injured mice

so as to illuminate the molecular mechanism of APS sheltering radiation injured haematogenesis.Methods:The injured mice were divided into 3 groups:normal control group

normal saline group

and APS group.Normal saline group and APS group were given NS or APS for 6 days continuously after the establishment of radiation injured mice model by X ray.Techniques of peripheral blood cells and BMNCs count were made.p53mRNA in murine BMNCs were assayed by RT-PCR.The cell cycle and cell apoptosis in BMNCs were detected by FCM(flow cytometry) on the 7th day.Results:Compare with those in NS group

the numbers of the peripheral leukocytes

erythrocytes

platelets and BMNCs in APS group increased significantly

while the expression of p53mRNA

the percentage of G0/G1 phases

and cell apoptosis rate obviously decreased.Conclusion:APS can decrease the the expression of p53mRNA

improve the chaos of cell cycle

suppress cell apoptosis in radiation injured mice

which can help the recovery of hematopoietic function in mice.

关键词

当归多糖小鼠放射损伤细胞周期凋亡

Keywords

angelica polysaccharides(APS)miceradiation injurycell cycleapoptosis

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