The cytoprotective effects of Lithium chloride on HUVECs through regulating fractalkine during the anoxia/reoxygenation

您当前的位置：

首页 >

文章列表页 >

The cytoprotective effects of Lithium chloride on HUVECs through regulating fractalkine during the anoxia/reoxygenation

更新时间：2024-01-17

|

- The cytoprotective effects of Lithium chloride on HUVECs through regulating fractalkine during the anoxia/reoxygenation
- Journal of Chongqing Medical University Vol. 36, Issue 7, Pages: 818-821(2011)
- 作者机构：
  
  重庆医科大学附属第一医院老年科
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2011.07.037
  CLC： R965
- Published：2011，
扫描看全文
[1]殷菱,李法琦.氯化锂调节趋化因子Fractalkine表达抑制缺氧复氧诱导的血管内皮细胞损伤[J].重庆医科大学学报,2011,36(07):818-821.

YIN Ling, LI Fa-qi. The cytoprotective effects of Lithium chloride on HUVECs through regulating fractalkine during the anoxia/reoxygenation. [J]. Journal of Chongqing Medical University 36(7):818-821(2011)
[1]殷菱,李法琦.氯化锂调节趋化因子Fractalkine表达抑制缺氧复氧诱导的血管内皮细胞损伤[J].重庆医科大学学报,2011,36(07):818-821. DOI： 10.13406/j.cnki.cyxb.2011.07.037.

YIN Ling, LI Fa-qi. The cytoprotective effects of Lithium chloride on HUVECs through regulating fractalkine during the anoxia/reoxygenation. [J]. Journal of Chongqing Medical University 36(7):818-821(2011) DOI： 10.13406/j.cnki.cyxb.2011.07.037.

摘要

目的:观察缺氧复氧诱导人脐静脉血管内皮细胞（Human umbillcal vein endothelial cells

HUVECs）损伤和趋化因子（Fractalkine

FKN）表达变化及氯化锂（Lithium chloride

LiCl）的干预效应。方法:体外培养HUVECs并随机分为对照组、缺氧复氧组和LiCl 5、10 mmol/L组。MTT法测定HUVECs的存活率

流式细胞术检测HUVECs凋亡率

免疫细胞荧光技术检测HUVECs的FKN蛋白表达

RT-PCR技术检测HUVECs的FKN mRNA表达。结果:与对照组比较

缺氧复氧组HUVECs存活率显著降低但凋亡率显著增高（P<0.01）且FKN mRNA和蛋白质表达也显著增高（P<0.01）;与缺氧复氧组比较

LiCl 5、10 mmol/L组HUVECs存活率显著增高但凋亡率显著降低（P<0.01）且FKNmRNA和蛋白质表达也明显降低（P<0.05）。结论:缺氧复氧上调FKN表达诱导HUVECs损伤（存活率降低、凋亡率增加）;LiCl预处理能够下调FKN表达

显著减轻缺氧复氧导致的HUVECs损伤。

Abstract

Objective:To investigate the cytoprotective effects of lithium chloride(LiCl) on human umbillcal vein endothelial cells(HUVECs) through regulating Fractalkine(FKN) during the anoxia/reoxygenation.Methods:HUVECs were cultivated and divided into four groups randomly(control group

anoxia/reoxygenation group

LiCl 5 mmol/L group

and LiCl 10 mmol/L group)

which were used to establish the anoxia/reoxygenation models.The cell viability was detected by MTT method.The apoptosis rate of HUVECs was examined by Flow cytometry.The mRNA expression of FKN was assessed by RT-PCR.The intracellular distribution and expression of FKN were determined by immunofluorescent staining.Results:Compared with control group

the apoptotic rate of HUVECs increased but their viability rate reduced significantly in anoxia/reoxygenation group(all P<0.01).The mRNA and protein expression of FKN were increased remarkably in anoxia/reoxygenation group compared to control group(P<0.01).Compared with anoxia/reoxygenation group

the survival rate of HUVECs was increased but their apoptotic rate as well as the expression of FKN mRNA and FKN protein reduced significantly in LiCl 5 mmol/L and 10 mmol/L groups(P<0.01 or P<0.05).Conclusions:The anoxia/reoxygenation induced the damage of HUVECs through upregulating FKN expression.LiCl pretreatment can protect HUVECs against anoxia/reoxygenation injury via inhibiting FKN expression.

关键词

缺氧复氧内皮细胞细胞凋亡趋化因子氯化锂

Keywords

anoxia/reoxygenationendothelial cellapoptosisfractalkinelithium chloride

references

0

Views

49

下载量

1

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Related Articles

Inhibitory effects of TIMP-3 on endothelial cell proliferation

Effect of Glycyrrhiza uralensis Fisch. endophytes combined with cisplatin on proliferation and apoptosis of A549 cells

Milk fat globule-EGF factor Ⅷ attenuates apoptosis and permeability damage of the intestinal mucosa in rats with obstructive jaundice

Effect of andrographolide on TBHP-induced apoptosis of intervertebral disc nucleus pulposus cells and its mechanism

Response of H₂O₂ stimulated human periodontal ligament cells to baicalin

Related Author

LI Shengwei

et al

王一如

刘长安

龚建平

李钺

李友伟

Zhang Shanglong

Related Institution

Department of Hepatobiliary Surgery,the Second Affiliated Hospital,Chongqing Medical University

重庆市肿瘤医院肿瘤研究所

重庆医科大学检验系

重庆医科大学附属第二医院肝胆外科

⁰