Heat shock protein inhibitor 17-AAG induces apoptosis of HNE1 cell line in human nasopharyngeal carcinoma

ZHAO Ju-mei1; LIU Tao2

doi:10.13406/j.cnki.cyxb.2011.07.033

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Heat shock protein inhibitor 17-AAG induces apoptosis of HNE1 cell line in human nasopharyngeal carcinoma

更新时间：2024-01-17

- Heat shock protein inhibitor 17-AAG induces apoptosis of HNE1 cell line in human nasopharyngeal carcinoma
- Journal of Chongqing Medical University Vol. 36, Issue 7, Pages: 782-785(2011)
- 作者机构：
  
  1. 延安大学医学院药理教研室
  2. 延安大学附属医院耳鼻咽喉科
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2011.07.033
  CLC： R739.63
- Published：2011，
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[1]赵菊梅,刘涛.17-丙烯胺基-17-去甲氧基格尔德霉素诱导鼻咽癌细胞凋亡的实验研究[J].重庆医科大学学报,2011,36(07):782-785.

ZHAO Ju-mei1, LIU Tao2. Heat shock protein inhibitor 17-AAG induces apoptosis of HNE1 cell line in human nasopharyngeal carcinoma. [J]. Journal of Chongqing Medical University 36(7):782-785(2011)
[1]赵菊梅,刘涛.17-丙烯胺基-17-去甲氧基格尔德霉素诱导鼻咽癌细胞凋亡的实验研究[J].重庆医科大学学报,2011,36(07):782-785. DOI： 10.13406/j.cnki.cyxb.2011.07.033.

ZHAO Ju-mei1, LIU Tao2. Heat shock protein inhibitor 17-AAG induces apoptosis of HNE1 cell line in human nasopharyngeal carcinoma. [J]. Journal of Chongqing Medical University 36(7):782-785(2011) DOI： 10.13406/j.cnki.cyxb.2011.07.033.

摘要

目的:研究17-丙烯胺基-17-去甲氧基格尔德霉素（17-allylamino-17-demethoxygeldanamycin

17-AAG）对人鼻咽癌细胞株（Human nasopharyngeal carcinoma cell line

HNE1）增殖抑制和诱导凋亡的作用

探讨17-AAG在鼻咽癌临床治疗中的意义。方法:采用噻唑蓝（Thiazolyl blue

MTT）检测17-AAG对HNE1细胞的增殖抑制作用

流式细胞仪检测细胞凋亡率的变化

Hoechst33528染色观察细胞的凋亡形态学变化并进行核碎片计数。结果:17-AAG抑制HNE1的增殖

诱导了细胞的凋亡

抑制作用具有浓度和时间依赖性。结论:17-AAG可诱导HNE1的凋亡

研究结果对药物治疗鼻咽癌有一定的临床指导意义。

Abstract

Objective:To observe the inhibitory effects of heat shock protein inhibitor 17-AAG on proliferation and induction of apoptosis of HNE1 cells in human nasopharyngeal carcinoma and investigate its clinical significance for the treatment of nasopharyngeal carcinoma.Methods:MTT reduction assay was used to measure cell viability.Flow cytometry was used to detect apoptotic cells.Hoechst33528 staining and fluorescence microscopy was used for morphological observation of apoptotic cells and calculation of nuclear fragmentations.Results:17-AAG inhibited proliferation and induce apoptosis of human nasopharyngeal carcinoma HNE1 cell line in vitro.The inhibition of proliferation was in time and dose dependent manner.Conclusion:17-AAG could induce human nasopharyngeal carcinoma cells to undergo apoptosis and can be a potential anti-cancer drug to treat nasopharyngeal carcinoma.

关键词

17-丙烯胺基-17-去甲氧基格尔德霉素细胞凋亡人鼻咽癌细胞株

Keywords

17-allylamino-17-demethoxygeldanamycinapoptosishuman nasopharyngeal carcinoma cell line

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