Expression of CDK13 mRNA in the brain tissue of patients with drug-resistant epilepsy

HAN Pu; WANG Xue-feng; WANG Liang

doi:10.13406/j.cnki.cyxb.2011.07.031

您当前的位置：

首页 >

文章列表页 >

Expression of CDK13 mRNA in the brain tissue of patients with drug-resistant epilepsy

更新时间：2024-01-17

- Expression of CDK13 mRNA in the brain tissue of patients with drug-resistant epilepsy
- Journal of Chongqing Medical University Vol. 36, Issue 7, Pages: 797-799(2011)
- 作者机构：
  
  重庆医科大学附属第一医院神经内科
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2011.07.031
  CLC： R742.1
- Published：2011，
扫描看全文
[1]韩璞,王学峰,王亮.CDK13在耐药性癫痫患者脑组织中的表达[J].重庆医科大学学报,2011,36(07):797-799.

HAN Pu, WANG Xue-feng, WANG Liang. Expression of CDK13 mRNA in the brain tissue of patients with drug-resistant epilepsy. [J]. Journal of Chongqing Medical University 36(7):797-799(2011)
[1]韩璞,王学峰,王亮.CDK13在耐药性癫痫患者脑组织中的表达[J].重庆医科大学学报,2011,36(07):797-799. DOI： 10.13406/j.cnki.cyxb.2011.07.031.

HAN Pu, WANG Xue-feng, WANG Liang. Expression of CDK13 mRNA in the brain tissue of patients with drug-resistant epilepsy. [J]. Journal of Chongqing Medical University 36(7):797-799(2011) DOI： 10.13406/j.cnki.cyxb.2011.07.031.

摘要

目的:研究耐药性癫痫患者脑组织内依赖细胞周期的蛋白激酶13（Cell cycle-dependent protein kinase 13

CDK13）mRNA的表达。方法:在基因芯片扫描的基础上

运用逆转录-多聚酶链反应（Reverse transcription polymerase chain reaction

RT-PCR）技术检测CDK 13mRNA在难治性癫痢患者脑部的变化。结果:基因芯片扫描显示CDK13 mRNA在耐药性癫痫患者脑部比正常人表达上调2.159倍

RT-PCR检测同样显示CDK13 mRNA在耐药性癫痫患者脑部表达增强

信号明显增高

提示候选基因CDK-13 mRNA在耐药性癫痫患者脑组织中表达上调

RT-PCR与cDNA芯片结果一致。结论:虽然CDK13具体生物功能尚不明确

但我们认为CDK13可能作为重要的调节因子参与了神经元凋亡等分子事件。CDK13mRNA表达上调可能在耐药性癫痫发病机制中起一定作用。

Abstract

Objective:To study the change of CDK13 mRNA expression in the brain tissue of patients with drug-resistant epilepsy so as to explore the possible mechanisms of the pathogenesis of refractory epilepsy.Methods: The microarray scanning and reverse transcription polymerase chain reaction(RT-PCR) were used to evaluate CDK-13mRNA expression in the brain tissue of 40 refractory epileptic patients and 8 normal controls.Results:As compared with the 8 normal controls

there appeared a significant increase in CDK13 mRNA expression in the patients with drug-resistant epilepsy

with the ratio of cy5/cy3 being 2.159 in microarray scanning(CDK13 mRNA expression in the patients with drug-resistant epilepsy was upregulated by 2.159 times compared with the normal controls.)

and the results of RT-PCR were coincided well with those of the cDNA chip.Conclusion: Further study should be made to clarify the precise function of CDK13.CDK13 may take part in the molecular event in neuronal apoptosis as an important modulator.The up-regulation of CDK13 mRNA expression may play an important role in the epileptogenic mechanisms of drug-resistant epilepsy

关键词

CDK13耐药性癫痫基因芯片逆转录-多聚酶链反应能量代谢

Keywords

CDK13drug-resistant epilepsycomplementary DNA microarray(cDNA microarray)RT-PCRenergy metabolism

references

Views

107

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Expression of Ngn3 in the donor islets with different purity

Protective effect of Shenfu injection on myocardial energy metabolism in septic mice

Expressions of ROCK1,TRAF3 mRNA in hippocampus tissues of the patients with drug-resistant epilepsy and its significance

Related Author

XUE Dong

et al

杨闯

尹昌生

LIU Qiong

et al

徐昉

曹友德

Related Institution

Departments of Hepatobiliary Surgery & Liver Transplantation,the First Affiliated Hospital,Chongqing Medical University

重庆医科大学附属第一医院肝胆暨肝移植外科

Department of Central Intensive Care Unit,the First Affiliated Hospital,Chongqing Medical University

重庆医科大学基础医学院病理学教研室

Department of Neurology,the First Afilliate Hospital,Chongqing Medical University

⁰