Study on electrophysiological characteristics of cardiomyocytes at different developmental stages from C57 mice by microelectrodes arrays

ZHANG Ling1; SUN Juan2; SHI Li3; HOU Yue-mei1

doi:10.13406/j.cnki.cyxb.2011.07.009

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Study on electrophysiological characteristics of cardiomyocytes at different developmental stages from C57 mice by microelectrodes arrays

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- Study on electrophysiological characteristics of cardiomyocytes at different developmental stages from C57 mice by microelectrodes arrays
- Journal of Chongqing Medical University Vol. 36, Issue 7, Pages: 836-841(2011)
- 作者机构：
  
  1. 新疆医科大学第一附属医院心律失常实验室
  2. 新疆自治区人民医院神经内科
  3. 中国人民解放军总医院肿瘤科
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2011.07.009
  CLC： R329
- Published：2011，
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[1]张玲,孙娟,时利,侯月梅.微电极阵列芯片技术研究不同发育阶段C57小鼠心肌细胞的电生理特性[J].重庆医科大学学报,2011,36(07):836-841.

ZHANG Ling1, SUN Juan2, SHI Li3, et al. Study on electrophysiological characteristics of cardiomyocytes at different developmental stages from C57 mice by microelectrodes arrays. [J]. Journal of Chongqing Medical University 36(7):836-841(2011)
[1]张玲,孙娟,时利,侯月梅.微电极阵列芯片技术研究不同发育阶段C57小鼠心肌细胞的电生理特性[J].重庆医科大学学报,2011,36(07):836-841. DOI： 10.13406/j.cnki.cyxb.2011.07.009.

ZHANG Ling1, SUN Juan2, SHI Li3, et al. Study on electrophysiological characteristics of cardiomyocytes at different developmental stages from C57 mice by microelectrodes arrays. [J]. Journal of Chongqing Medical University 36(7):836-841(2011) DOI： 10.13406/j.cnki.cyxb.2011.07.009.

摘要

目的:微电极阵列芯片技术研究不同发育阶段C57小鼠心肌细胞的电生理特性。方法:分离13.5

9.5 d胎鼠和2 d乳鼠的心室肌细胞并培养于微电极阵列（Microelectrode arrays

MEA）电极盘中48 h

通过倒置相差显微镜

透射电镜、扫描电镜观察其形态

免疫组化鉴定并用MEA技术研究其电生理特性。并对生后1、7、14、21、28、35、

60 d的意识清醒未麻醉的小鼠行心电图（Electrocardiogram

ECG）和心率变异性（Heart rate variability

HRV）记录。结果:胎鼠心室肌细胞场电位时程（Field poten-tial duration

FPd）随发育而增加（P

0.01）

乳鼠的突然明显缩短（P

0.01）。胎鼠心室肌细胞的搏动频率随发育而减慢。出生后小鼠的心律是窦性心律

平均PR、RR、QRS、QT、JT间期随发育逐渐缩短

7 d组比1 d组减少的差异有统计学意义（P

0.01）

而14、21、28、35和

60 d之间差异无统计学意义。J-S-T段在1 d小鼠明显抬高

7 d组和14 d组逐渐降低

到35 d则基本回到基线。HRV随发育逐渐增加。结论:C57小鼠胎鼠心肌细胞FP电生理特性的变化和生后小鼠ECG间期和形态随发育的变化可能为小鼠心脏随发育的电生理特性提供有用的信息。

Abstract

Objective:To explore electrophysiological characteristics of cardiomyocytes at different developmental stages from C57 mice by microelectrodes arrays.Methods: The single ventricular cardiomyocytes from fetal in 13.5~19.5 d and neonatal in 2 d C57 mice were obtained and cultured in MEA(microelectrode arrays)plates about 48 h respectively

and to be observed through the methods of inverted phase contrast microscope

transmission electron microscope

scanning electron microscope

and identified through immunocytochemistry and MEA which investigated the electrophysiological properties.ECGs and heart rate variability(HRV)were recorded in conscious

unsedated C57 mice at different postnatal days(days 1

>60).Results: The field potential duration prolonged with the increase of gestational age in cultured ventricular cardiomyocytes(P<0.01)

then decreased in neonatal(P<0.01).And the beat frequence decreased with development in ventricular cardiomyocytes from embryotal mice.In postnatal mice

the heart rhythm was sinus rhythm

mean PR

QRS

JT interval decreased with development

there was significant difference on day 1 and day 7(P< 0.01)

and without significant difference on day 14

35 and > 60.The J-S-T segment was elevated in day 1 neonatal

resolved by day 14 and disappeared in 35 day.The HRV increased with development.Conclusion:The field potential properties of embryonic cardiomyocytes and ECG intervals and morphological changes during the normal postnatal mice with development may provide some useful information for exploration the development of electrophysiology characteristics in heart from C57 mice.

关键词

微电极阵列小鼠发育电生理特性

Keywords

microelectrode arraymicedevelopmentelectrophysiology characteristics

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